|
rdf:type |
|
|
lifeskim:mentions |
umls-concept:C0006675,
umls-concept:C0016030,
umls-concept:C0020291,
umls-concept:C0030685,
umls-concept:C0035820,
umls-concept:C0040018,
umls-concept:C0205102,
umls-concept:C0242606,
umls-concept:C0332291,
umls-concept:C0391871,
umls-concept:C0680255,
umls-concept:C0684180,
umls-concept:C1283071,
umls-concept:C1698986,
umls-concept:C1963578
|
|
pubmed:issue |
1-2
|
|
pubmed:dateCreated |
1999-9-21
|
|
pubmed:abstractText |
V79 fibroblasts were treated with ultraviolet (UV) C radiation alone as well as in conjunction with chronic oxidative stress. The effects of these treatments on calcium signaling were observed at 30 min post-irradiation. In the absence of extracellular calcium, thrombin released calcium from internal stores of UVC-irradiated V79 fibroblasts even after exposure to neomycin. In neomycin-treated control and chronic oxidative stress cells, no calcium release by thrombin was observed after chelation of external calcium. Calcium release by thrombin from internal stores of UV-irradiated and neomycin-treated cells was completely abolished by pretreatment with N-acetyl cysteine and dexamethasone. Cellular total soluble thiol content which is a good indicator of cellular reduced glutathione (GSH) level was significantly elevated 30 min after ultraviolet radiation, indicating an adaptive response after oxidative stress. Chronic oxidative stress alone resulted in a much smaller increase in GSH but chronic oxidative stress in conjunction with UVC produced a very prominent elevation in GSH levels. Our data suggest that thrombin can cause calcium release from internal stores of ultraviolet-irradiated fibroblasts which is independent of phosphatidylinositol bisphosphate hydrolysis and is directly related to the level of oxidative stress. Involvement of phospholipase A2 and a role for its products as possible mediators of calcium release from intracellular stores, is strongly indicated.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jun
|
|
pubmed:issn |
0300-8177
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
196
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
23-30
|
|
pubmed:dateRevised |
2009-11-19
|
|
pubmed:meshHeading |
pubmed-meshheading:10448899-Animals,
pubmed-meshheading:10448899-Calcium,
pubmed-meshheading:10448899-Calcium-Calmodulin-Dependent Protein Kinases,
pubmed-meshheading:10448899-Cells, Cultured,
pubmed-meshheading:10448899-Cricetinae,
pubmed-meshheading:10448899-Cricetulus,
pubmed-meshheading:10448899-Cyclooxygenase Inhibitors,
pubmed-meshheading:10448899-Fibroblasts,
pubmed-meshheading:10448899-Genistein,
pubmed-meshheading:10448899-Glycogen Synthase Kinase 3,
pubmed-meshheading:10448899-Hydrolysis,
pubmed-meshheading:10448899-Lipoxygenase Inhibitors,
pubmed-meshheading:10448899-Lung,
pubmed-meshheading:10448899-Oxidative Stress,
pubmed-meshheading:10448899-Phosphatidylinositol 4,5-Diphosphate,
pubmed-meshheading:10448899-Sulfhydryl Compounds,
pubmed-meshheading:10448899-Thrombin,
pubmed-meshheading:10448899-Ultraviolet Rays
|
|
pubmed:year |
1999
|
|
pubmed:articleTitle |
Thrombin releases calcium from internal stores of ultraviolet C-treated V79 fibroblasts independent of phosphatidylinositol bisphosphate hydrolysis: role of oxidative stress.
|
|
pubmed:affiliation |
Crystallography & Molecular Biology Division, Saha Institute of Nuclear Physics, Calcutta, India.
|
|
pubmed:publicationType |
Journal Article
|
