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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1999-9-23
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pubmed:databankReference |
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pubmed:abstractText |
Placental leucine aminopeptidase (P-LAP) which is identical with cystine aminopeptidase as oxytocinase was found to be a homologue of rat insulin-regulated membrane aminopeptidase (IRAP) by cDNA cloning. In this study, we confirmed 5'-end cDNA sequence of P-LAP and isolated genomic clones containing the upstream region of human P-LAP gene. The transcription initiation sites determined by primer extension located 478 and 480 bp upstream of the initiation methionine codon, 38 bp downstream of TATA box-like motif. The 5'-flanking region of human P-LAP gene contained DNA-binding motifs for several ubiquitous transcription factors such as SP1 and AP2. Chromosomal localization by fluorescence in situ hybridization showed that the gene was assigned to 5q14.2-q15 of the human chromosome. This study establishes the genetic basis for P-LAP gene research, thereby leading to better understanding of the molecular mechanism underlying the P-LAP gene.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0006-291X
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pubmed:author |
pubmed-author:HorioJJ,
pubmed-author:KatsumataYY,
pubmed-author:KinoshitaMM,
pubmed-author:KumanoYY,
pubmed-author:MizutaniSS,
pubmed-author:NakanishiYY,
pubmed-author:NakazatoHH,
pubmed-author:NomuraSS,
pubmed-author:OkadaMM,
pubmed-author:TakamiSS,
pubmed-author:TsujimotoMM
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pubmed:copyrightInfo |
Copyright 1999 Academic Press.
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pubmed:issnType |
Print
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pubmed:day |
19
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pubmed:volume |
262
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
269-74
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pubmed:dateRevised |
2009-11-24
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pubmed:meshHeading |
pubmed-meshheading:10448104-Aminopeptidases,
pubmed-meshheading:10448104-Animals,
pubmed-meshheading:10448104-Base Sequence,
pubmed-meshheading:10448104-Chromosomes, Human, Pair 5,
pubmed-meshheading:10448104-Cloning, Molecular,
pubmed-meshheading:10448104-Codon, Initiator,
pubmed-meshheading:10448104-Cystinyl Aminopeptidase,
pubmed-meshheading:10448104-Genomic Library,
pubmed-meshheading:10448104-Humans,
pubmed-meshheading:10448104-In Situ Hybridization, Fluorescence,
pubmed-meshheading:10448104-Molecular Sequence Data,
pubmed-meshheading:10448104-Physical Chromosome Mapping,
pubmed-meshheading:10448104-Placenta,
pubmed-meshheading:10448104-Promoter Regions, Genetic,
pubmed-meshheading:10448104-RNA, Messenger,
pubmed-meshheading:10448104-Rats,
pubmed-meshheading:10448104-Response Elements,
pubmed-meshheading:10448104-Sequence Alignment,
pubmed-meshheading:10448104-TATA Box
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pubmed:year |
1999
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pubmed:articleTitle |
Structural organization of the 5'-end and chromosomal assignment of human placental leucine aminopeptidase/insulin-regulated membrane aminopeptidase gene.
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pubmed:affiliation |
Department of Obstetrics and Gynecology, Nagoya University School of Medicine, Nagoya, 466-8550, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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