Source:http://linkedlifedata.com/resource/pubmed/id/10446449
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
6
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pubmed:dateCreated |
1999-9-10
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pubmed:abstractText |
Several types of lipoxygenases with various functions occur in mammalian cells. Although the presence of 12-lipoxygenase activity has been reported in uterine tissues, neither its type nor its biological functions have yet been established. Moreover, the putative role of uterine 12-lipoxygenase in cervical cancer has not been addressed before. Homogenates of uterine tissues from women without cancer and from patients with invasive cervical carcinoma were incubated with (1-(14)C)-arachidonic acid under various conditions and the labelled reaction products were analyzed both by thin-layer chromatography and by high-pressure liquid chromatography. 12-Lipoxygenase protein was estimated by Western blot using anti-serum against recombinant human platelet-type 12-lipoxygenase. Highest concentrations and activities of 12-lipoxygenase were found in the exocervix. The formation of 12S-hydroxy-5Z,8Z,10E, 14Z-eicosatetraenoic acid (12-HETE) was stimulated by micromolar concentrations of 13S-hydroperoxy-9Z,11E-octadecadienoic acid, suggesting metabolic control of the 12-lipoxygenase activity via the hydroperoxide tone. Immunohistochemical investigation revealed that the enzyme is mainly located in the squamous epithelium, and is of platelet-type. Significantly lower values for the 12-HETE formation were found in samples from patients with invasive cervical carcinoma, whereas the amount of immunochemically detectable 12-lipoxygenase protein was unaltered. At the same time the expression levels of the bcl-2 gene were enhanced. Thus, it is concluded that during carcinogenesis the hydroperoxide-reducing capacity of the uterine cervix tissue is enhanced, possibly mediated by bcl-2 protein, and in turn metabolically suppresses the 12-lipoxygenase activity. Furthermore, the data suggest an anti-carcinogenic action of 12-lipoxygenase in human cervix, in contrast to its reported pro-carcinogenic action in breast cancer.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0020-7136
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pubmed:author | |
pubmed:copyrightInfo |
Copyright 1999 Wiley-Liss, Inc.
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pubmed:issnType |
Print
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pubmed:day |
9
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pubmed:volume |
82
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
827-31
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pubmed:dateRevised |
2007-7-24
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pubmed:meshHeading |
pubmed-meshheading:10446449-12-Hydroxy-5,8,10,14-eicosatetraenoic Acid,
pubmed-meshheading:10446449-Arachidonate 12-Lipoxygenase,
pubmed-meshheading:10446449-Blood Platelets,
pubmed-meshheading:10446449-Cervix Uteri,
pubmed-meshheading:10446449-Endometrium,
pubmed-meshheading:10446449-Female,
pubmed-meshheading:10446449-Genes, bcl-2,
pubmed-meshheading:10446449-Humans,
pubmed-meshheading:10446449-Myometrium,
pubmed-meshheading:10446449-Neoplasm Invasiveness,
pubmed-meshheading:10446449-Substrate Specificity,
pubmed-meshheading:10446449-Uterine Neoplasms
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pubmed:year |
1999
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pubmed:articleTitle |
Metabolic suppression of platelet-type 12-lipoxygenase in human uterine cervix with invasive carcinoma.
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pubmed:affiliation |
Eicosanoid Research Division, Gynaecology Department, Benjamin Franklin University Medical Centre, Free University Berlin, Berlin, Germany. nigam@zedat.fu-berlin.de
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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