Source:http://linkedlifedata.com/resource/pubmed/id/10446203
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
34
|
| pubmed:dateCreated |
1999-9-9
|
| pubmed:databankReference | |
| pubmed:abstractText |
A CCAAT box-binding protein subunit, CBF-C/NF-YC, was cloned as a protein involved in the c-Myc complex formed on the G(1)-specific enhancer in the human hsp70 gene. CBF-C/NF-YC directly bound to c-Myc in vitro and in vivo in cultured cells. The CBF/NF-Y.c-Myc complex required the HSP-MYC-B element as well as CCAAT in the hsp70 G(1)-enhancer, while the purified CBF subunits recognized only CCAAT even in the presence of c-Myc. Both the HSP-MYC-B and CCAAT elements were also required for the enhancer activity. In transient transfection experiments, the CBF/NF-Y.c-Myc complex, as well as transcription due to the G(1)-enhancer, was increased by the introduction of c-Myc at low doses but decreased at high doses. The repression of both complex formation and transcription by c-Myc at high doses was abrogated by the introduction of CBF/NF-Y in a dose-dependent manner. Furthermore, the CBF/NF-Y.c-Myc complex bound to the G(1)-enhancer appeared in the early G(1) phase of the cell cycle when c-Myc was not higly expressed and gradually disappeared after the c-Myc expression reached its maximum. The results indicate that the cell cycle-dependent expression of the hsp70 gene is regulated by the intracellular amount of c-Myc through the complex formation states between CBF/NF-Y and c-Myc.
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| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/CCAAT-Enhancer-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/HSP70 Heat-Shock Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-myc
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Aug
|
| pubmed:issn |
0021-9258
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
20
|
| pubmed:volume |
274
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
24270-9
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:10446203-3T3 Cells,
pubmed-meshheading:10446203-Animals,
pubmed-meshheading:10446203-Base Sequence,
pubmed-meshheading:10446203-CCAAT-Enhancer-Binding Proteins,
pubmed-meshheading:10446203-CHO Cells,
pubmed-meshheading:10446203-Cell Cycle,
pubmed-meshheading:10446203-Cricetinae,
pubmed-meshheading:10446203-DNA,
pubmed-meshheading:10446203-DNA-Binding Proteins,
pubmed-meshheading:10446203-Gene Expression Regulation,
pubmed-meshheading:10446203-HSP70 Heat-Shock Proteins,
pubmed-meshheading:10446203-Humans,
pubmed-meshheading:10446203-Mice,
pubmed-meshheading:10446203-Molecular Sequence Data,
pubmed-meshheading:10446203-Promoter Regions, Genetic,
pubmed-meshheading:10446203-Proto-Oncogene Proteins c-myc,
pubmed-meshheading:10446203-Rabbits,
pubmed-meshheading:10446203-Transcription, Genetic
|
| pubmed:year |
1999
|
| pubmed:articleTitle |
Cell cycle-dependent switch of up-and down-regulation of human hsp70 gene expression by interaction between c-Myc and CBF/NF-Y.
|
| pubmed:affiliation |
Graduate School of Pharmaceutical Sciences, Hokkaido University, Kita-ku, Sapporo 060, Japan.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|