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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
8
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pubmed:dateCreated |
1999-8-30
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pubmed:abstractText |
A novel metal-binding site has been identified in the hammerhead ribozyme by 31P NMR. The metal-binding site is associated with the A13 phosphate in the catalytic core of the hammerhead ribozyme and is distinct from any previously identified metal-binding sites. 31P NMR spectroscopy was used to measure the metal-binding affinity for this site and leads to an apparent dissociation constant of 250-570 microM at 25 degrees C for binding of a single Mg2+ ion. The NMR data also show evidence of a structural change at this site upon metal binding and these results are compared with previous data on metal-induced structural changes in the core of the hammerhead ribozyme. These NMR data were combined with the X-ray structure of the hammerhead ribozyme (Pley HW, Flaherty KM, McKay DB. 1994. Nature 372:68-74) to model RNA ligands involved in binding the metal at this A13 site. In this model, the A13 metal-binding site is structurally similar to the previously identified A(g) metal-binding site and illustrates the symmetrical nature of the tandem G x A base pairs in domain 2 of the hammerhead ribozyme. These results demonstrate that 31P NMR represents an important method for both identification and characterization of metal-binding sites in nucleic acids.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/10445883-1383927,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10445883-1716459,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10445883-2036380,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10445883-2235484,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10445883-239391,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10445883-3684574,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10445883-6509032,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10445883-7511031,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10445883-7541315,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10445883-7719853,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10445883-7969422,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10445883-8241158,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10445883-8290345,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10445883-8752086,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10445883-8917508,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10445883-8942631,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10445883-8953035,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10445883-9042941,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10445883-9204421,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10445883-9214652,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10445883-9341112,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10445883-9405376,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10445883-9417029,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10445883-9506521,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/10445883-9922178
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
1355-8382
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
5
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1099-104
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:10445883-Base Sequence,
pubmed-meshheading:10445883-Binding Sites,
pubmed-meshheading:10445883-Dose-Response Relationship, Drug,
pubmed-meshheading:10445883-Ions,
pubmed-meshheading:10445883-Magnesium,
pubmed-meshheading:10445883-Magnetic Resonance Spectroscopy,
pubmed-meshheading:10445883-Models, Genetic,
pubmed-meshheading:10445883-Models, Molecular,
pubmed-meshheading:10445883-Molecular Sequence Data,
pubmed-meshheading:10445883-Phosphates,
pubmed-meshheading:10445883-RNA, Catalytic
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pubmed:year |
1999
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pubmed:articleTitle |
Identification and characterization of a novel high affinity metal-binding site in the hammerhead ribozyme.
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pubmed:affiliation |
Department of Chemistry and Biochemistry, University of Colorado at Boulder, 80309-0215, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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