Source:http://linkedlifedata.com/resource/pubmed/id/10444741
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
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| pubmed:dateCreated |
1999-9-17
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| pubmed:abstractText |
Actinobacillus actinomycetemcomitans is a major periodontal pathogen which is associated with both early-onset periodontitis and adult cases refractory to conventional periodontal therapy, although the organism has also been shown to be widely distributed among dentate healthy individuals. The observed disease status may be associated with a variation in virulence of different strains or clones. The aim of the present study was to analyse genotype distribution as assessed by an arbitrarily primed polymerase chain reaction (AP-PCR) among 51 isolates of A. actinomycetemcomitans recovered from more than 200 young adult recruits with no or minor periodontal disease. In addition, isolates from 25 periodontitis patients as well as reference strains were genotyped. Primers amplifying (i) a specific sequence in the ltxA region, (ii) a specific 16S rRNA sequence and (iii) sequences in the leukotoxin promoter region were used to verify species identity of the strains. Three random oligonucleotide primers were employed to analyse genomic polymorphisms of the organism by means of PCR. A total of 19 genotypes could be distinguished, which were grouped by cluster analysis into 5 major clusters based on genetic similarity and a complete linkage sort. Whereas 3 clusters assembled A. actinomycetemcomitans genotypes isolated from both healthy subjects and periodontitis patients, one cluster containing 4 different genotypes exclusively comprised isolates from healthy or gingivitis subjects. Another cluster with 2 genotypes consisted of strains originating from periodontitis patients (p < 0.05). One strain characterized by a specific 530 bp deletion in the promoter region of the leukotoxin region was identified in a Ghanese patient with localized juvenile periodontitis. It was concluded that there is considerable clonal diversity of A. actinomycetemcomitans strains isolated from healthy or periodontally diseased subjects, and that genetically closely related groups might be associated with health or disease.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
D
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Toxins,
http://linkedlifedata.com/resource/pubmed/chemical/Cytotoxins,
http://linkedlifedata.com/resource/pubmed/chemical/Exotoxins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Ribosomal, 16S,
http://linkedlifedata.com/resource/pubmed/chemical/leukotoxin
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| pubmed:status |
MEDLINE
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| pubmed:month |
May
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| pubmed:issn |
0022-3484
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
34
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
179-87
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| pubmed:dateRevised |
2010-11-18
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| pubmed:meshHeading |
pubmed-meshheading:10444741-Actinobacillus Infections,
pubmed-meshheading:10444741-Actinobacillus actinomycetemcomitans,
pubmed-meshheading:10444741-Adolescent,
pubmed-meshheading:10444741-Adult,
pubmed-meshheading:10444741-Aggressive Periodontitis,
pubmed-meshheading:10444741-Bacterial Toxins,
pubmed-meshheading:10444741-Base Pairing,
pubmed-meshheading:10444741-Clone Cells,
pubmed-meshheading:10444741-Cytotoxins,
pubmed-meshheading:10444741-Exotoxins,
pubmed-meshheading:10444741-Gene Deletion,
pubmed-meshheading:10444741-Genetic Linkage,
pubmed-meshheading:10444741-Genotype,
pubmed-meshheading:10444741-Gingivitis,
pubmed-meshheading:10444741-Humans,
pubmed-meshheading:10444741-Male,
pubmed-meshheading:10444741-Periodontitis,
pubmed-meshheading:10444741-Periodontium,
pubmed-meshheading:10444741-Polymerase Chain Reaction,
pubmed-meshheading:10444741-Polymorphism, Genetic,
pubmed-meshheading:10444741-Promoter Regions, Genetic,
pubmed-meshheading:10444741-RNA, Ribosomal, 16S,
pubmed-meshheading:10444741-Virulence
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| pubmed:year |
1999
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| pubmed:articleTitle |
Clonal diversity of Actinobacillus actinomycetemcomitans isolates from young adults with minimal periodontal disease.
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| pubmed:affiliation |
Central Institute of the German Armed Forces Medical Service, Koblenz, Germany.
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| pubmed:publicationType |
Journal Article
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