Linked Life Data

10444485

Source:http://linkedlifedata.com/resource/pubmed/id/10444485

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2 Pt 2
pubmed:dateCreated
1999-9-23
pubmed:abstractText
Formamide-induced osmotic shock has been used to detubulate isolated adult rat ventricular myocytes (i.e., disrupt the surface membrane-T tubule junction). Cell volume, calculated from cell length and width, rapidly decreased and increased upon application and removal of formamide, respectively. After treatment with formamide, membrane capacitance decreased by 26.4% (from 199.4 +/- 18.7 pF in control cells to 146.7 +/- 6.4 pF in formamide-treated cells; n = 13, P < 0.05). However, the amplitude of the L-type Ca(2+) current (I(Ca)) decreased by a greater extent (from 0.75 +/- 0.14 to 0.18 +/- 0.03 nA; n = 5, P < 0.05) so that the density of I(Ca) decreased by 74.5%. Simultaneous measurements of I(Ca) and Ca(2+) transients (monitored using fura 2) showed that both decreased rapidly upon removal of formamide. However, the Ca(2+) content of the sarcoplasmic reticulum showed little change. Cross-striations, visualized with the fluorescent dye di-8-aminonaphthylethenylpyridinium, were sparse or absent in cells that had been treated with formamide, suggesting that formamide can successfully detubulate cardiac cells and that I(Ca) is concentrated in the T tubules, which therefore play an important role in excitation-contraction coupling.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0002-9513
pubmed:author
pubmed:issnType
Print
pubmed:volume
277
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
H603-9
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1999
pubmed:articleTitle
Excitation-contraction coupling in rat ventricular myocytes after formamide-induced detubulation.
pubmed:affiliation
School of Biomedical Sciences, University of Leeds, Leeds LS2 9NQ, United Kingdom.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't