Linked Life Data

10444469

Source:http://linkedlifedata.com/resource/pubmed/id/10444469

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2 Pt 2
pubmed:dateCreated
1999-9-23
pubmed:abstractText
A mode of action of endothelin (ET) on spontaneous contractions was investigated in ring preparations of isolated bovine mesenteric lymphatics. ET-1 at concentrations between 10(-10) and 10(-9) M caused a dose-dependent increase in the frequency of spontaneous contractions. The specific ET(A)-receptor antagonist BQ-123 (5 x 10(-7) M) caused a significant inhibition of the ET-1-induced positive chronotropic effect in the ring preparations with and without the endothelium. Mechanical denudation of the lymphatic endothelial cells produced a significant potentiation of the ET-induced positive chronotropic effect. BQ-3020 (10(-8)-10(-7) M), a selective ET(B)-receptor agonist, induced dose dependently negative chronotropic and inotropic effects on the spontaneous contractions in the ring preparations with intact endothelium. Mechanical removal of the endothelium caused a significant reduction of the BQ-3020-induced negative chronotropic and inotropic effects. The ET-1-induced positive chronotropic effect was potentiated by pretreatment with N(omega)-nitro-L-arginine methyl ester (L-NAME) (10(-5) M) but unaffected by aspirin (10(-5) M). Additional treatment with L-arginine (10(-4) M) completely reversed the L-NAME-mediated potentiation of the ET-induced chronotropic effect. These results suggest that stimulation of ET(A) receptors on the lymphatic smooth muscles causes a positive chronotropic effect on the spontaneous contractions, and stimulation of ET(B) receptors on the lymphatic endothelial cells induces a release of nitric oxide, which results in the chronotropic and inotropic effects on spontaneous contractions in isolated bovine mesenteric lymphatics.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0002-9513
pubmed:author
pubmed:issnType
Print
pubmed:volume
277
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
H459-66
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:10444469-Animals, pubmed-meshheading:10444469-Arginine, pubmed-meshheading:10444469-Aspirin, pubmed-meshheading:10444469-Cattle, pubmed-meshheading:10444469-Chronobiology Phenomena, pubmed-meshheading:10444469-Dose-Response Relationship, Drug, pubmed-meshheading:10444469-Drug Combinations, pubmed-meshheading:10444469-Drug Synergism, pubmed-meshheading:10444469-Endothelin-1, pubmed-meshheading:10444469-Endothelins, pubmed-meshheading:10444469-Enzyme Inhibitors, pubmed-meshheading:10444469-Lymphatic System, pubmed-meshheading:10444469-Mesentery, pubmed-meshheading:10444469-Muscle, Smooth, pubmed-meshheading:10444469-Muscle Contraction, pubmed-meshheading:10444469-NG-Nitroarginine Methyl Ester, pubmed-meshheading:10444469-Peptide Fragments, pubmed-meshheading:10444469-Peptides, Cyclic
pubmed:year
1999
pubmed:articleTitle
Effects of endothelin on spontaneous contractions in lymph vessels.
pubmed:affiliation
The First Department of Physiology, Shinshu University School of Medicine, Matsumoto 390-8621, Japan.
pubmed:publicationType
Journal Article, In Vitro