Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2 Pt 1
pubmed:dateCreated
1999-9-23
pubmed:abstractText
Sustained smooth muscle contraction is mediated by protein kinase C (PKC) through a signal transduction cascade leading to contraction. Heat-shock protein 27 (HSP27) appears to be the link between these two major events, i.e., signal transduction and sustained smooth muscle contraction. We have investigated the involvement of HSP27 in signal transduction and HSP27 association with contractile proteins (e.g., actin, myosin, tropomyosin, and caldesmon) resulting in sustained smooth muscle contraction. We have carried out confocal microscopy to investigate the cellular reorganization and colocalization of proteins and immunoprecipitation of HSP27 with actin, myosin, tropomyosin, and caldesmon as detected by sequential immunoblotting. Our results indicate that 1) translocation of Raf-1 to the membrane when stimulated with ceramide is inhibited by vasoactive intestinal peptide (VIP), a relaxant neuropeptide; 2) PKC-alpha and mitogen-activated protein kinase translocate and colocalize on the membrane in response to ceramide, and PKC-alpha translocation is inhibited by VIP; 3) HSP27 colocalizes with actin when contraction occurs; and 4) HSP27 immunoprecipitates with actin and with the contractile proteins myosin, tropomyosin, and caldesmon. We propose a model in which HSP27 is involved in sustained smooth muscle contraction and modulates the interaction of actin, myosin, tropomyosin, and caldesmon.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Actins, http://linkedlifedata.com/resource/pubmed/chemical/Azepines, http://linkedlifedata.com/resource/pubmed/chemical/Calcium-Calmodulin-Dependent..., http://linkedlifedata.com/resource/pubmed/chemical/Calmodulin-Binding Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Ceramides, http://linkedlifedata.com/resource/pubmed/chemical/Contractile Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Heat-Shock Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Isoenzymes, http://linkedlifedata.com/resource/pubmed/chemical/ML 7, http://linkedlifedata.com/resource/pubmed/chemical/Myosins, http://linkedlifedata.com/resource/pubmed/chemical/Naphthalenes, http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase C, http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase C-alpha, http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-raf, http://linkedlifedata.com/resource/pubmed/chemical/Tropomyosin
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0002-9513
pubmed:author
pubmed:issnType
Print
pubmed:volume
277
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
G445-54
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:10444459-Actins, pubmed-meshheading:10444459-Animals, pubmed-meshheading:10444459-Azepines, pubmed-meshheading:10444459-Biological Transport, pubmed-meshheading:10444459-Calcium-Calmodulin-Dependent Protein Kinases, pubmed-meshheading:10444459-Calmodulin-Binding Proteins, pubmed-meshheading:10444459-Ceramides, pubmed-meshheading:10444459-Colon, pubmed-meshheading:10444459-Contractile Proteins, pubmed-meshheading:10444459-Cytoskeleton, pubmed-meshheading:10444459-Heat-Shock Proteins, pubmed-meshheading:10444459-Isoenzymes, pubmed-meshheading:10444459-Muscle, Smooth, pubmed-meshheading:10444459-Muscle Contraction, pubmed-meshheading:10444459-Myosins, pubmed-meshheading:10444459-Naphthalenes, pubmed-meshheading:10444459-Precipitin Tests, pubmed-meshheading:10444459-Protein Kinase C, pubmed-meshheading:10444459-Protein Kinase C-alpha, pubmed-meshheading:10444459-Proto-Oncogene Proteins c-raf, pubmed-meshheading:10444459-Rabbits, pubmed-meshheading:10444459-Signal Transduction, pubmed-meshheading:10444459-Tissue Distribution, pubmed-meshheading:10444459-Tropomyosin
pubmed:year
1999
pubmed:articleTitle
HSP27 in signal transduction and association with contractile proteins in smooth muscle cells.
pubmed:affiliation
Department of Pediatrics, University of Michigan Medical Center, Ann Arbor, Michigan 48109, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.