Linked Life Data

10441760

Source:http://linkedlifedata.com/resource/pubmed/id/10441760

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1999-9-16
pubmed:abstractText
Here we investigate differences in the temporal and spatial patterning, and subunit interactions of two of the major Na(+) channel alpha-subunit isoforms in mammalian brain, the type I and type II Na(+) channels. By using subtype-specific antibodies, we find that both isoforms are abundant in adult rat brain, where both interact with the covalently bound beta2 auxiliary subunit. Immunoblot analysis reveals complementary levels of type I and type II in different brain regions, with the highest levels of type I in brainstem, cortex, substantia nigra, and caudate, where it is found predominantly on the soma of neurons, and the highest levels of type II in globus pallidus, hippocampus and thalamus, where it is preferentially localized to axons. Developmentally, type I Na(+) channel polypeptide expression in brain increases dramatically during the third postnatal week, peaks at the end of the first postnatal month, and then decreases such that adult levels are approximately 50% of those at peak. Type II Na(+) channel polypeptide expression in brain also undergoes large increases in the third postnatal week, but levels continue to increase such that peak expression levels are maintained in adult animals. Type I Na(+) channels are found associated with the auxiliary beta2 subunit at all ages, whereas free type II Na(+) channels exist during the first two postnatal weeks. Thus, although expression of these two Na(+) channel alpha subunits in heterologous systems yields currents with very similar electrophysiological and pharmacological properties, their distinct spatial and temporal patterning, and association with auxiliary subunits in brain, suggest that they perform distinct, nonoverlapping functions in situ.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0021-9967
pubmed:author
pubmed:copyrightInfo
Copyright 1999 Wiley-Liss, Inc.
pubmed:issnType
Print
pubmed:day
20
pubmed:volume
412
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
342-52
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:10441760-Aging, pubmed-meshheading:10441760-Amino Acid Sequence, pubmed-meshheading:10441760-Anesthetics, Local, pubmed-meshheading:10441760-Animals, pubmed-meshheading:10441760-Animals, Newborn, pubmed-meshheading:10441760-Antibodies, pubmed-meshheading:10441760-Brain, pubmed-meshheading:10441760-Brain Chemistry, pubmed-meshheading:10441760-Immunohistochemistry, pubmed-meshheading:10441760-Macromolecular Substances, pubmed-meshheading:10441760-Molecular Sequence Data, pubmed-meshheading:10441760-Neurons, pubmed-meshheading:10441760-Organ Specificity, pubmed-meshheading:10441760-Peptide Fragments, pubmed-meshheading:10441760-Protein Isoforms, pubmed-meshheading:10441760-Pyramidal Cells, pubmed-meshheading:10441760-Pyramidal Tracts, pubmed-meshheading:10441760-Rats, pubmed-meshheading:10441760-Sodium Channels
pubmed:year
1999
pubmed:articleTitle
Type I and type II Na(+) channel alpha-subunit polypeptides exhibit distinct spatial and temporal patterning, and association with auxiliary subunits in rat brain.
pubmed:affiliation
Department of Biochemistry and Cell Biology and Institute for Cell and Developmental Biology, State University of New York, Stony Brook, New York 11794-5215, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't