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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1999-9-9
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pubmed:abstractText |
In the present studies, we demonstrate that heregulin is a potent and rapid activator of the serine/threonine kinase called Akt in the MCF-7 breast cancer cell line but not in 3 other breast cancer cell lines (T47D, HBL-100, and MDA-231). The extent of activation of Akt in the 4 cell lines correlated with the ability of heregulin to activate phosphatidylinositol 3-kinase and inhibition of the kinase blocked Akt activation. A monoclonal antibody to HER2 inhibited the ability of heregulin to activate Akt in the MCF-7 cells. BT474, a breast cancer cell line which overexpresses HER2, had high basal Akt enzymatic activity. This high basal activity was lowered when cells were pre-incubated with an anti-HER2 monoclonal antibody which is used to treat breast cancer patients. Our results indicate that heregulin is a potent activator of Akt and that overexpression of HER2 in breast cancers could also lead to activation of Akt.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/AKT1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Neuregulin-1,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylinositol 3-Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Serine-Threonine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-akt,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, Epidermal Growth Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, erbB-2,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, erbB-3,
http://linkedlifedata.com/resource/pubmed/chemical/heregulin beta1
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 1999 Academic Press.
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pubmed:issnType |
Print
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pubmed:day |
11
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pubmed:volume |
261
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
897-903
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:10441522-Breast Neoplasms,
pubmed-meshheading:10441522-Carrier Proteins,
pubmed-meshheading:10441522-Enzyme Activation,
pubmed-meshheading:10441522-Gene Expression,
pubmed-meshheading:10441522-Glycoproteins,
pubmed-meshheading:10441522-Humans,
pubmed-meshheading:10441522-Neuregulin-1,
pubmed-meshheading:10441522-Phosphatidylinositol 3-Kinases,
pubmed-meshheading:10441522-Protein-Serine-Threonine Kinases,
pubmed-meshheading:10441522-Proto-Oncogene Proteins,
pubmed-meshheading:10441522-Proto-Oncogene Proteins c-akt,
pubmed-meshheading:10441522-Receptor, Epidermal Growth Factor,
pubmed-meshheading:10441522-Receptor, erbB-2,
pubmed-meshheading:10441522-Receptor, erbB-3,
pubmed-meshheading:10441522-Tumor Cells, Cultured
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pubmed:year |
1999
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pubmed:articleTitle |
Heregulin regulation of Akt/protein kinase B in breast cancer cells.
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pubmed:affiliation |
Department of Molecular Pharmacology, Stanford University School of Medicine, Stanford, California, 94305, USA.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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