Linked Life Data

10438976

Source:http://linkedlifedata.com/resource/pubmed/id/10438976

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1999-9-9
pubmed:abstractText
Memory T cells that home to inflamed tissues typically express the beta-chemokine receptor CCR5 and exhibit a Th1 cytokine profile. The migration of these cells into the genital tract following antigenic exposure has particular relevance to acquisition of HIV-1 infection, because CCR5 functions as the coreceptor for most sexually transmitted HIV-1 strains. We recently established methodology to purify and culture mononuclear cells from the female reproductive tract, and here we analyzed the phenotype, CCR5 expression, and cytokine production of cervicovaginal T cells in up to 16 donors. The proportion of mucosal T cells expressing CCR5 was markedly expanded as compared with peripheral blood (mean 88% vs 24% in 13 donors), but the receptor density on individual CCR5+ T cells was only slightly increased (mean 5837 vs 4191 MEPE (molecules of equivalent PE) units in 6 of 7 donors). Intracellular costaining for IL-2, IFN-gamma, IL-4, and IL-5 revealed a Th1-type pattern in cervical T cells, with significantly higher percentages of IL-2- and IFN-gamma-producing T cells in the mucosa than in blood (mean 67% vs 29%). Coexpression of surface CCR5 with intracellular IL-2 and IFN-gamma was observed only among T cells in the mucosa, but not among those in circulation. Thus, we postulate that T cell homing to the genital mucosa leads to differentiation into the combined CCR5+ Th1 phenotype. Moreover, the predominance of CCR5+ Th1-type T cells in normal cervical mucosa provides targets accessible for the efficient transmission of macrophage-tropic HIV-1 variants in women following sexual exposure.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
163
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2306-13
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed-meshheading:10438976-Antigens, CD, pubmed-meshheading:10438976-Antigens, CD45, pubmed-meshheading:10438976-Antigens, Differentiation, T-Lymphocyte, pubmed-meshheading:10438976-Cell Separation, pubmed-meshheading:10438976-Cervix Uteri, pubmed-meshheading:10438976-Female, pubmed-meshheading:10438976-Humans, pubmed-meshheading:10438976-Immunophenotyping, pubmed-meshheading:10438976-Interferon-gamma, pubmed-meshheading:10438976-Interleukin-2, pubmed-meshheading:10438976-Lectins, C-Type, pubmed-meshheading:10438976-Mucous Membrane, pubmed-meshheading:10438976-Receptors, Antigen, T-Cell, alpha-beta, pubmed-meshheading:10438976-Receptors, CCR5, pubmed-meshheading:10438976-Receptors, Lymphocyte Homing, pubmed-meshheading:10438976-T-Lymphocyte Subsets, pubmed-meshheading:10438976-Th1 Cells, pubmed-meshheading:10438976-Vagina
pubmed:year
1999
pubmed:articleTitle
Coexpression of CCR5 and IL-2 in human genital but not blood T cells: implications for the ontogeny of the CCR5+ Th1 phenotype.
pubmed:affiliation
Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't