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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
33
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pubmed:dateCreated |
1999-9-1
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pubmed:databankReference |
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pubmed:abstractText |
We have identified a novel human zinc finger protein, hFOG-2, which is related to but distinct from the murine transcription factor Friend-of-GATA-1 (mFOG-1). The hFOG-2 gene was initially detected in K562 cells using a polymerase chain reaction approach with degenerate primers corresponding to zinc finger regions of mFOG-1. A murine homologue of hFOG-2 was also identified in the mouse expressed sequence tag data banks, indicating that a family of FOG genes exists in mammals. hFOG-2 appears to be widely expressed, while mFOG-1 is expressed primarily in erythroid and megakaryocytic cells and plays a fundamental role in the development of these lineages. Sequencing of the full-length hFOG-2 cDNA indicates that the interaction domains for transcription factors GATA-1 and mCtBP2 are both conserved and we have shown that this new FOG protein also physically interacts with these factors. We have demonstrated that hFOG-2, like mFOG-1, can act in concert with GATA-1 to activate gene expression from the p45 NF-E2 promoter region, but that it can also act to repress GATA-mediated activation of additional reporter constructs. Finally, we have identified a repression domain in hFOG-2 and show that repression is dependent upon the integrity of the mCtBP2 interaction motif Pro-Ile-Asp-Leu-Ser.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Ctbp2 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Erythroid-Specific DNA-Binding...,
http://linkedlifedata.com/resource/pubmed/chemical/GATA1 Transcription Factor,
http://linkedlifedata.com/resource/pubmed/chemical/GATA1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Gata1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/NF-E2 Transcription Factor,
http://linkedlifedata.com/resource/pubmed/chemical/NF-E2 Transcription Factor, p45...,
http://linkedlifedata.com/resource/pubmed/chemical/NFE2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Nfe2 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Repressor Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/ZFPM2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Zfpm2 protein, mouse
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
13
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pubmed:volume |
274
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
23491-8
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:10438528-Amino Acid Sequence,
pubmed-meshheading:10438528-Animals,
pubmed-meshheading:10438528-Base Sequence,
pubmed-meshheading:10438528-Cell Line,
pubmed-meshheading:10438528-Cloning, Molecular,
pubmed-meshheading:10438528-DNA Primers,
pubmed-meshheading:10438528-DNA-Binding Proteins,
pubmed-meshheading:10438528-Erythroid-Specific DNA-Binding Factors,
pubmed-meshheading:10438528-GATA1 Transcription Factor,
pubmed-meshheading:10438528-Humans,
pubmed-meshheading:10438528-Mice,
pubmed-meshheading:10438528-Molecular Sequence Data,
pubmed-meshheading:10438528-NF-E2 Transcription Factor,
pubmed-meshheading:10438528-NF-E2 Transcription Factor, p45 Subunit,
pubmed-meshheading:10438528-Phosphoproteins,
pubmed-meshheading:10438528-Promoter Regions, Genetic,
pubmed-meshheading:10438528-Protein Binding,
pubmed-meshheading:10438528-Repressor Proteins,
pubmed-meshheading:10438528-Sequence Homology, Amino Acid,
pubmed-meshheading:10438528-Transcription Factors,
pubmed-meshheading:10438528-Zinc Fingers
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pubmed:year |
1999
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pubmed:articleTitle |
hFOG-2, a novel zinc finger protein, binds the co-repressor mCtBP2 and modulates GATA-mediated activation.
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pubmed:affiliation |
Centre for Thrombosis and Vascular Research, School of Pathology, University of New South Wales, Sydney, New South Wales 2052, Australia.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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