Source:http://linkedlifedata.com/resource/pubmed/id/10438362
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
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pubmed:dateCreated |
1999-9-23
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pubmed:abstractText |
A pair of isogenic, nontypeable Haemophilus influenzae strains, one expressing protein D and the other protein D-negative, was compared in their ability to cause damage in a human nasopharyngeal tissue culture model. Damage was assessed by measuring the ciliary beat frequency (CBF) of tissue specimens at 12 h intervals. Cultures inoculated with H. influenzae manifested a decrease in CBF beginning after 12 h, with a maximum decrease after 36 h. The impairment of ciliary function by the protein D-expressing strain was significantly greater than that caused by the protein D-negative mutant (P<.01). Tissue specimens examined by scanning and transmission electron microscopy after 24 h appeared normal. After 48 h of incubation, the protein D-expressing strain caused a significant loss of cilia. These findings suggest that protein D is involved in the pathogenesis of upper respiratory tract infections due to nontypeable H. influenzae, probably by enhancing functional and morphological damage to cilia.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin D,
http://linkedlifedata.com/resource/pubmed/chemical/Lipoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/glpQ protein, Haemophilus influenzae
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0022-1899
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
180
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
737-46
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:10438362-Adenoids,
pubmed-meshheading:10438362-Bacterial Proteins,
pubmed-meshheading:10438362-Carrier Proteins,
pubmed-meshheading:10438362-Cilia,
pubmed-meshheading:10438362-Epithelial Cells,
pubmed-meshheading:10438362-Haemophilus influenzae,
pubmed-meshheading:10438362-Humans,
pubmed-meshheading:10438362-Immunoglobulin D,
pubmed-meshheading:10438362-Lipoproteins,
pubmed-meshheading:10438362-Microscopy, Electron,
pubmed-meshheading:10438362-Microscopy, Electron, Scanning,
pubmed-meshheading:10438362-Nasopharynx,
pubmed-meshheading:10438362-Organ Culture Techniques
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pubmed:year |
1999
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pubmed:articleTitle |
Effects on the ciliated epithelium of protein D-producing and -nonproducing nontypeable Haemophilus influenzae in nasopharyngeal tissue cultures.
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pubmed:affiliation |
Department of Medical Microbiology, Lund University, University Hospital, Malmö, Sweden.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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