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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2-4
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pubmed:dateCreated |
1999-8-3
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pubmed:abstractText |
The effects of IL-12 on the responses of cattle peripheral blood mononuclear cells (PBMC) to bovine respiratory syncytial virus (BRSV) antigen and ovalbumin (OVA) were tested, in vitro. IL-12 did not affect the proliferative responses of PBMC to these antigens but markedly accelerated and augmented the level of IFNgamma secreted. When tested on lymphoblasts rather than resting T-cells IL-12 also enhanced proliferation. In contrast IL-4 and, to greater extent, IL-10 inhibited the response. The effect of IL-12 on IFNgamma synthesis was confirmed at the level of IFNgamma. mRNA expression using Taqman PCR. CD4 and CD8 T-cell populations produced IFNgamma, however, CD4 T-cells comprised the largest contributors to the IFNgamma production. Gamma/delta T-cells did not contribute markedly. A comparison of the species cross-reactivity showed bovine IL-12 was also active in the human system. This study shows that antigen-driven responses in cattle can be significantly influenced by exogenous cytokines and suggests the IL-12/IL-10 balance is crucial for regulation of IFNgamma.
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0165-2427
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
68
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
193-207
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:10438320-Animals,
pubmed-meshheading:10438320-CD4-Positive T-Lymphocytes,
pubmed-meshheading:10438320-CD8-Positive T-Lymphocytes,
pubmed-meshheading:10438320-Cattle,
pubmed-meshheading:10438320-Cross Reactions,
pubmed-meshheading:10438320-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:10438320-Humans,
pubmed-meshheading:10438320-Interferon-gamma,
pubmed-meshheading:10438320-Interleukin-10,
pubmed-meshheading:10438320-Interleukin-12,
pubmed-meshheading:10438320-Interleukin-4,
pubmed-meshheading:10438320-Lymphocyte Activation,
pubmed-meshheading:10438320-Mice,
pubmed-meshheading:10438320-Ovalbumin,
pubmed-meshheading:10438320-Polymerase Chain Reaction,
pubmed-meshheading:10438320-RNA, Messenger,
pubmed-meshheading:10438320-Respiratory Syncytial Virus, Bovine,
pubmed-meshheading:10438320-Species Specificity
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pubmed:year |
1999
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pubmed:articleTitle |
Bovine interleukin-12 and modulation of IFNgamma production.
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pubmed:affiliation |
Division of Immunology and Pathology, Institute for Animal Health, Newbury, Berkshire, UK. collinsr@bbsrc.ac.uk
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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