Source:http://linkedlifedata.com/resource/pubmed/id/10435731
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1999-10-8
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pubmed:abstractText |
Interleukin-12 (IL-12) is a crucial cytokine regulating cell-mediated immunity, and may contribute to the development of graft-versus-host disease (GVHD). We investigated serum IL-12 concentrations, interferon gamma (IFN-gamma) production by peripheral blood lymphocytes (PBL) from allogeneic stem cell recipients after IL-12 plus anti-CD3 monoclonal antibody (mAb) stimulation. We also investigated IL-12 production by peripheral macrophages (Mphi) after lipopolysaccharide (LPS) stimulation from allogeneic stem cell recipients and patients receiving donor leukocyte transfusions (DLT) for treatment or prophylaxis of leukemia relapse and Epstein-Barr virus (EBV) lymphoproliferative disease (LPD). PBL from acute GVHD patients produced high IFN-gamma levels after IL-12 plus anti-CD3 mAb stimulation, whereas PBL from patients without acute GVHD produced low levels of IFN-gamma. However, serum IL-12 concentrations were low in both groups. Peripheral Mphi IL-12 production increased in patients who developed acute GVHD compared to patients without acute GVHD. Five patients receiving DLT for treatment or prophylaxis of leukemia relapse developed acute GVHD. IFN-gamma production by PBL stimulated by IL-12 plus anti-CD3 mAb increased, while IL-12 production by peripheral Mphi stimulated by LPS was very high after the development of acute GVHD. However, serum IL-12 concentration remained low. Three patients receiving DLT for EBV-LPD did not develop acute GVHD with no increase of IFN-gamma and IL-12 production. These results indicate that IL-12 may play an important role in the development of acute GVHD after allogeneic stem cell grafting or DLT, and increased IL-12 production by Mphi occurs with various stimuli, including LPS.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0268-3369
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
24
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
29-34
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:10435731-Acute Disease,
pubmed-meshheading:10435731-Adolescent,
pubmed-meshheading:10435731-Adult,
pubmed-meshheading:10435731-Bone Marrow Transplantation,
pubmed-meshheading:10435731-Child,
pubmed-meshheading:10435731-Child, Preschool,
pubmed-meshheading:10435731-Female,
pubmed-meshheading:10435731-Graft vs Host Disease,
pubmed-meshheading:10435731-Humans,
pubmed-meshheading:10435731-Infant,
pubmed-meshheading:10435731-Interferon-gamma,
pubmed-meshheading:10435731-Interleukin-12,
pubmed-meshheading:10435731-Leukemia,
pubmed-meshheading:10435731-Lipopolysaccharides,
pubmed-meshheading:10435731-Lymphocytes,
pubmed-meshheading:10435731-Macrophages,
pubmed-meshheading:10435731-Male
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pubmed:year |
1999
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pubmed:articleTitle |
Role of interleukin-12 in the development of acute graft-versus-host disease in bone marrow transplant patients.
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pubmed:affiliation |
Department of Pediatrics, Tokai University School of Medicine, Kanagawa, Isehara, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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