Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
28
pubmed:dateCreated
1999-8-13
pubmed:abstractText
Our previous allelic loss analysis of gamma-ray induced thymic lymphomas in F1 hybrid and backcross mice between BALB/c and MSM strains mapped the Tlsr4 region exhibiting a high frequency of allelic loss (62%) to a 2.9 cM interval between the markers D12Mit53 and D12Mit279 on mouse chromosome 12. To narrow further the interval harboring a putative tumor suppressor gene, a high-density scan has been carried out for informative 361 thymic lymphomas. Construction of a physical map of Tlsr4 with 3 YAC and 15 BAC clones and isolation of YAC- and BAC-derived polymorphic probes lead to fine allelic loss mapping. Three successive polymorphic sites within one BAC exhibit the retention of both alleles in seven, one and four lymphomas, suggesting that a common region of allelic loss for Tlsr4 exists within the BAC region. Pulsed-field gel electrophoresis of NotI digests of this and other clones determines that the commonly lost region is a 35 kb interval with a NotI site. NotI sites are frequently associated with coding regions, and our preliminary sequencing has identified ESTs in the region. Thus, the present study facilitates the identification of genes in the Tlsr4 region that would lead to isolation of a novel tumor suppressor gene.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0950-9232
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
18
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4131-6
pubmed:dateRevised
2008-8-23
pubmed:meshHeading
pubmed-meshheading:10435594-Alleles, pubmed-meshheading:10435594-Animals, pubmed-meshheading:10435594-Chromosome Mapping, pubmed-meshheading:10435594-Chromosomes, Artificial, Yeast, pubmed-meshheading:10435594-Cloning, Molecular, pubmed-meshheading:10435594-Crosses, Genetic, pubmed-meshheading:10435594-Deoxyribonucleases, Type II Site-Specific, pubmed-meshheading:10435594-Electrophoresis, Gel, Pulsed-Field, pubmed-meshheading:10435594-Expressed Sequence Tags, pubmed-meshheading:10435594-Gamma Rays, pubmed-meshheading:10435594-Gene Deletion, pubmed-meshheading:10435594-Genes, Tumor Suppressor, pubmed-meshheading:10435594-Genes, p53, pubmed-meshheading:10435594-Genetic Markers, pubmed-meshheading:10435594-Genetic Vectors, pubmed-meshheading:10435594-Loss of Heterozygosity, pubmed-meshheading:10435594-Lymphoma, T-Cell, pubmed-meshheading:10435594-Mice, pubmed-meshheading:10435594-Mice, Inbred BALB C, pubmed-meshheading:10435594-Mice, Inbred Strains, pubmed-meshheading:10435594-Neoplasms, Radiation-Induced, pubmed-meshheading:10435594-Polymorphism, Restriction Fragment Length, pubmed-meshheading:10435594-Thymus Neoplasms, pubmed-meshheading:10435594-Tumor Suppressor Protein p53
pubmed:year
1999
pubmed:articleTitle
Allelic loss mapping and physical delineation of a region harboring a putative thymic lymphoma suppressor gene on mouse chromosome 12.
pubmed:affiliation
Department of Biochemistry, Niigata University School of Medicine, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't