Linked Life Data

10435587

Source:http://linkedlifedata.com/resource/pubmed/id/10435587

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
28
pubmed:dateCreated
1999-8-13
pubmed:abstractText
The insulin-like growth factor II receptor (IGFIIR) has been implicated as a tumor suppressor gene in human malignancy. Frequent mutation, loss of heterozygosity, and microsatellite instability (MSI) directly affecting the IGFIIR gene have been reported in several primary human tumor types. However, to our knowledge, dynamic functional evidence of a growth-suppressive role for IGFIIR has not yet been provided. We identified one MSI-positive colorectal carcinoma cell line, SW48, with monoallelic mutation in IGFIIR identical to that seen in primary colorectal carcinomas. A zinc-inducible construct containing the wild-type IGFIIR cDNA was stably transfected into SW48 cells. Growth rate and apoptosis were compared between zinc-treated, untreated, and untransfected cells. A twofold increase in IGFIIR protein expression was detected after zinc treatment in discrete clonal isolates of transfected SW48 cells. Moreover, zinc induction of exogenous wild-type IGFIIR expression reproducibly decreased growth rate and increased apoptosis. These data prove that wild-type IGFIIR functions as a growth suppressor gene in colorectal cancer cells and provide dynamic in vitro functional support for the hypothesis that IGFIIR is a human growth suppressor gene.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0950-9232
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
18
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4063-8
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:10435587-Adenocarcinoma, pubmed-meshheading:10435587-Apoptosis, pubmed-meshheading:10435587-Cell Division, pubmed-meshheading:10435587-Colorectal Neoplasms, pubmed-meshheading:10435587-DNA, Complementary, pubmed-meshheading:10435587-Gene Expression Regulation, Neoplastic, pubmed-meshheading:10435587-Humans, pubmed-meshheading:10435587-Insulin-Like Growth Factor II, pubmed-meshheading:10435587-Loss of Heterozygosity, pubmed-meshheading:10435587-Metallothionein, pubmed-meshheading:10435587-Microsatellite Repeats, pubmed-meshheading:10435587-Neoplasm Proteins, pubmed-meshheading:10435587-Polymerase Chain Reaction, pubmed-meshheading:10435587-Promoter Regions, Genetic, pubmed-meshheading:10435587-Receptor, IGF Type 2, pubmed-meshheading:10435587-Tumor Cells, Cultured, pubmed-meshheading:10435587-Zinc
pubmed:year
1999
pubmed:articleTitle
Expression of the wild-type insulin-like growth factor II receptor gene suppresses growth and causes death in colorectal carcinoma cells.
pubmed:affiliation
Department of Medicine, Dallas VA Medical Center and University of Texas-Southwestern Medical School, 75216, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't