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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1999-8-17
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pubmed:abstractText |
We describe the construction of retroviral plasmid vectors in which two genes are linked by a minimum of 96 nucleotides encoding the 2A sequence from the picornavirus foot-and-mouth disease virus (FMDV). Transcription and translation gives rise to a bicistronic mRNA and two independent protein products. The system offers advantages to other alternative ways to create polycistronic mRNAs and can be used in gene therapy delivery vectors.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0969-7128
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
6
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
198-208
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:10435104-3T3 Cells,
pubmed-meshheading:10435104-Acetyltransferases,
pubmed-meshheading:10435104-Animals,
pubmed-meshheading:10435104-Antiviral Agents,
pubmed-meshheading:10435104-Aphthovirus,
pubmed-meshheading:10435104-Blotting, Western,
pubmed-meshheading:10435104-Chimera,
pubmed-meshheading:10435104-Ganciclovir,
pubmed-meshheading:10435104-Gene Expression,
pubmed-meshheading:10435104-Gene Therapy,
pubmed-meshheading:10435104-Genetic Vectors,
pubmed-meshheading:10435104-Genome, Viral,
pubmed-meshheading:10435104-Humans,
pubmed-meshheading:10435104-Mice,
pubmed-meshheading:10435104-Plasmids,
pubmed-meshheading:10435104-Thymidine Kinase,
pubmed-meshheading:10435104-Tumor Cells, Cultured
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pubmed:year |
1999
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pubmed:articleTitle |
Use of the 2A sequence from foot-and-mouth disease virus in the generation of retroviral vectors for gene therapy.
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pubmed:affiliation |
Departamento de Bioquímica y Biología Molecular, Universidad Autónoma de Madrid, Facultad de Ciencias, Spain.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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