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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
31
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pubmed:dateCreated |
1999-9-3
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pubmed:abstractText |
Basic peptides from the carboxy terminus of the HIV-1 Tat protein bind to the apical stem-loop region of TAR RNA with high affinity and moderate specificity. The conformations of the unbound and 24 residue Tat peptide (Tfr24)-bound forms of TAR RNA have been characterized by NMR spectroscopy. The unbound form of TAR exists in major and minor forms having different trinucleotide bulge conformations. A specific TAR RNA conformational change is observed upon complex formation with Tfr24, consisting of coaxial stacking of helical stems and base triple formation. A U23-A27-U38 base triple is proposed based on exchangeable proton NMR data, where U23 forms a base pair with A27 in the major groove. No evidence for base triple formation was found for Tat peptides in which lysine residues are extensively substituted for arginine.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0006-2960
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
3
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pubmed:volume |
38
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
10059-69
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:10433713-Amino Acid Sequence,
pubmed-meshheading:10433713-Base Composition,
pubmed-meshheading:10433713-Gene Products, tat,
pubmed-meshheading:10433713-HIV Long Terminal Repeat,
pubmed-meshheading:10433713-HIV-1,
pubmed-meshheading:10433713-Humans,
pubmed-meshheading:10433713-Hydrogen Bonding,
pubmed-meshheading:10433713-Models, Chemical,
pubmed-meshheading:10433713-Models, Molecular,
pubmed-meshheading:10433713-Molecular Sequence Data,
pubmed-meshheading:10433713-Nuclear Magnetic Resonance, Biomolecular,
pubmed-meshheading:10433713-Nucleic Acid Conformation,
pubmed-meshheading:10433713-Peptide Fragments,
pubmed-meshheading:10433713-Protein Binding,
pubmed-meshheading:10433713-Protein Conformation,
pubmed-meshheading:10433713-RNA, Viral,
pubmed-meshheading:10433713-Solutions,
pubmed-meshheading:10433713-tat Gene Products, Human Immunodeficiency Virus
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pubmed:year |
1999
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pubmed:articleTitle |
Characterization of the solution conformations of unbound and Tat peptide-bound forms of HIV-1 TAR RNA.
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pubmed:affiliation |
Department of Chemistry, Yale University, New Haven, Connecticut 06520-8107, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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