Source:http://linkedlifedata.com/resource/pubmed/id/10431246
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
1999-8-26
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| pubmed:abstractText |
A physical map of the mouse genome is an essential tool for both positional cloning and genomic sequencing in this key model system for biomedical research. Indeed, the construction of a mouse physical map with markers spaced at an average interval of 300 kb is one of the stated goals of the Human Genome Project. Here we report the results of a project at the Whitehead Institute/MIT Center for Genome Research to construct such a physical map of the mouse. We built the map by screening sequenced-tagged sites (STSs) against a large-insert yeast artificial chromosome (YAC) library and then integrating the STS-content information with a dense genetic map. The integrated map shows the location of 9,787 loci, providing landmarks with an average spacing of approximately 300 kb and affording YAC coverage of approximately 92% of the mouse genome. We also report the results of a project at the MRC UK Mouse Genome Centre targeted at chromosome X. The project produced a YAC-based map containing 619 loci (with 121 loci in common with the Whitehead map and 498 additional loci), providing especially dense coverage of this sex chromosome. The YAC-based physical map directly facilitates positional cloning of mouse mutations by providing ready access to most of the genome. More generally, use of this map in addition to a newly constructed radiation hybrid (RH) map provides a comprehensive framework for mouse genomic studies.
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| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Aug
|
| pubmed:issn |
1061-4036
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| pubmed:author |
pubmed-author:AltF LFL,
pubmed-author:AndersonM OMO,
pubmed-author:BirrenB WBW,
pubmed-author:BoguskiM SMS,
pubmed-author:BrownS DSD,
pubmed-author:CadmanMM,
pubmed-author:CarterN PNP,
pubmed-author:CasaisMM,
pubmed-author:CollymoreA JAJ,
pubmed-author:CourtneyD FDF,
pubmed-author:DennyPP,
pubmed-author:DevineRR,
pubmed-author:DietrichW FWF,
pubmed-author:FarinoGG,
pubmed-author:GrayDD,
pubmed-author:HarrisK LKL,
pubmed-author:HaynesA RAR,
pubmed-author:HeustonCC,
pubmed-author:HortonL TLTJr,
pubmed-author:HudsonT JTJ,
pubmed-author:HusainZZ,
pubmed-author:JogN VNV,
pubmed-author:KouyoumjianVV,
pubmed-author:LanderE SES,
pubmed-author:MakalowskiWW,
pubmed-author:MarslandTT,
pubmed-author:MerportOO,
pubmed-author:MillerJJ,
pubmed-author:NagyFF,
pubmed-author:NusbaumCC,
pubmed-author:RossM TMT,
pubmed-author:SlonimD KDK,
pubmed-author:SouthwellAA,
pubmed-author:SteenR GRG,
pubmed-author:SteinL DLD,
pubmed-author:StrivensM AMA,
pubmed-author:TatMM,
pubmed-author:TrickettPP,
pubmed-author:WankRR,
pubmed-author:WuYY,
pubmed-author:XuYY,
pubmed-author:ZemstevaI SIS
|
| pubmed:issnType |
Print
|
| pubmed:volume |
22
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
388-93
|
| pubmed:dateRevised |
2009-9-29
|
| pubmed:meshHeading |
pubmed-meshheading:10431246-Animals,
pubmed-meshheading:10431246-Chromosome Mapping,
pubmed-meshheading:10431246-Chromosomes, Artificial, Yeast,
pubmed-meshheading:10431246-Contig Mapping,
pubmed-meshheading:10431246-Genetic Markers,
pubmed-meshheading:10431246-Genome,
pubmed-meshheading:10431246-Mice,
pubmed-meshheading:10431246-Models, Genetic,
pubmed-meshheading:10431246-Physical Chromosome Mapping
|
| pubmed:year |
1999
|
| pubmed:articleTitle |
A YAC-based physical map of the mouse genome.
|
| pubmed:affiliation |
Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|