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rdf:type |
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lifeskim:mentions |
umls-concept:C0035696,
umls-concept:C0040711,
umls-concept:C0179400,
umls-concept:C0242767,
umls-concept:C0600436,
umls-concept:C1186763,
umls-concept:C1314939,
umls-concept:C1519249,
umls-concept:C1552130,
umls-concept:C1627358,
umls-concept:C2349975
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pubmed:issue |
16
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pubmed:dateCreated |
1999-9-9
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pubmed:abstractText |
The downstream box (DB) is a sequence element that enhances translation of several bacterial and phage mRNAs. It has been proposed that the DB enhances translation by base pairing transiently to bases 1469-1483 of 16S rRNA, the so-called anti-DB, during the initiation phase of translation. We have tested this model of enhancer action by constructing mutations in the anti-DB that alter its mRNA base-pairing potential and examining expression of a variety of DB-containing mRNAs in strains expressing the mutant anti-DB 16S rRNA. We found that the rRNA mutant was viable and that expression of all tested DB-containing mRNAs was completely unaffected by radical alterations in the proposed anti-DB. These findings lead us to conclude that enhancement of translation by the DB does not involve mRNA-rRNA base pairing.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/10430880-10051579,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10430880-1107998,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10430880-1375310,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10430880-1380697,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10430880-1386558,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10430880-1507217,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10430880-1681509,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10430880-1700254,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10430880-1960727,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10430880-1961716,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10430880-2070415,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10430880-2186363,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10430880-2200518,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10430880-2440027,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10430880-2440028,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10430880-2643095,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10430880-2657653,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10430880-2676996,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10430880-3047677,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10430880-3053173,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10430880-3881765,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10430880-6319233,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10430880-7534475,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10430880-7559409,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10430880-7566085,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10430880-7678344,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10430880-7844820,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10430880-7966326,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10430880-803646,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10430880-8070416,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10430880-8287975,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10430880-8599950,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10430880-8722008,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10430880-8850007,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10430880-8887565,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10430880-8930918,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10430880-8972775,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10430880-9140962,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10430880-9149228,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10430880-9190796,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10430880-9190815,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10430880-9383157,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10430880-9547274,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10430880-9770676
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
3
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pubmed:volume |
96
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
8973-8
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:10430880-Escherichia coli,
pubmed-meshheading:10430880-Models, Molecular,
pubmed-meshheading:10430880-Base Sequence,
pubmed-meshheading:10430880-Protein Biosynthesis,
pubmed-meshheading:10430880-RNA, Messenger,
pubmed-meshheading:10430880-Bacterial Proteins,
pubmed-meshheading:10430880-Nucleic Acid Conformation,
pubmed-meshheading:10430880-Base Pairing,
pubmed-meshheading:10430880-Plasmids,
pubmed-meshheading:10430880-RNA, Ribosomal, 16S,
pubmed-meshheading:10430880-Transcription Factors,
pubmed-meshheading:10430880-Sigma Factor,
pubmed-meshheading:10430880-Heat-Shock Proteins,
pubmed-meshheading:10430880-Mutagenesis, Site-Directed,
pubmed-meshheading:10430880-Enhancer Elements, Genetic
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