Linked Life Data

10426504

Source:http://linkedlifedata.com/resource/pubmed/id/10426504

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1999-10-8
pubmed:abstractText
The localization of 5-hydroxytryptamine2C receptors in the lumbosacral spinal cord of the rat was investigated using selective antibodies raised against the carboxyl-terminal part of the rat receptor. The distribution of immunoperoxidase labelling at the light microscope level revealed numerous labelled neurons in the gray matter, with a higher intensity in the sacral parasympathetic nucleus, the dorsal gray commissure and particularly the motoneurons of the ventral horn. Confocal microscope analysis showed that immunostaining was mainly intracellular (motoneurons), but could also be associated with the membrane of cell bodies and dendrites. Actually, electron microscope immunogold experiments demonstrated an exclusive staining of the cis-Golgi apparatus. Following pseudo-rabies virus transsynaptic retrograde labelling from the corpus cavernosum, labelled neurons were found in the sacral parasympathetic nucleus and the dorsal gray commissure of the L6-S1 segments. All virus-labelled neurons exhibited 5-hydroxytryptamine2C receptor immunoreactivity. These results indicate that all parasympathetic preganglionic neurons and their related interneurons which contribute to the innervation of cavernosal tissue bear 5-hydroxytryptamine2C receptors. In the sacral parasympathetic nucleus, most neurons which were retrogradely-labelled from the pelvic ganglion with Fast Blue also showed 5-hydroxytryptamine2C receptor immunoreactivity. In the ventral horn, motoneurons retrogradely labelled from the ischiocavernosus muscle and the bulbospongiosus muscle, both of which are involved in erection and ejaculation, were also 5-hydroxytryptamine2C receptor-immunopositive. The supraspinal serotoninergic control of erection at the lumbosacral level therefore appears to be strongly associated with the activation of 5-hydroxytryptamine2C receptors, consistent with the proerectile properties of 5-hydroxytryptamine2C agonists.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0306-4522
pubmed:author
pubmed:issnType
Print
pubmed:volume
92
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1523-37
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:10426504-Amidines, pubmed-meshheading:10426504-Animals, pubmed-meshheading:10426504-Antibodies, Blocking, pubmed-meshheading:10426504-Coloring Agents, pubmed-meshheading:10426504-Herpesvirus 1, Suid, pubmed-meshheading:10426504-Histocytochemistry, pubmed-meshheading:10426504-Immunohistochemistry, pubmed-meshheading:10426504-Male, pubmed-meshheading:10426504-Microscopy, Confocal, pubmed-meshheading:10426504-Motor Neurons, pubmed-meshheading:10426504-Neurons, pubmed-meshheading:10426504-Parasympathetic Nervous System, pubmed-meshheading:10426504-Penile Erection, pubmed-meshheading:10426504-Penis, pubmed-meshheading:10426504-Rats, pubmed-meshheading:10426504-Rats, Sprague-Dawley, pubmed-meshheading:10426504-Receptor, Serotonin, 5-HT2C, pubmed-meshheading:10426504-Receptors, Serotonin, pubmed-meshheading:10426504-Serotonin Antagonists, pubmed-meshheading:10426504-Spinal Cord
pubmed:year
1999
pubmed:articleTitle
5-Hydroxytryptamine2C receptors on spinal neurons controlling penile erection in the rat.
pubmed:affiliation
Département de Neurobiologie des Signaux Intercellulaires, Institut des Neurosciences, CNRS UMR 7624, Université Pierre et Marie Curie, Paris, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't