Linked Life Data

10423574

Source:http://linkedlifedata.com/resource/pubmed/id/10423574

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1999-9-2
pubmed:abstractText
Light is emitted in systems containing N-chloramines or hypochlorite, H(2)O(2) and N-(4-aminobutyl)-N-ethylisoluminol (ABEI). The emission is enhanced by 4-iodophenol (PIP) in alkaline solution (1 mol/L NaOH), while at lower pH range (9-11) PIP is not only inactive but also its presence reduces chemiluminescence (CL) of the monochloramine-H(2)O(2)-ABEI system to the background. Two procedures for ABEI-labelled IgG assays were developed, with PIP in 1 mol/L NaOH and without PIP at pH 11, and the standard curves of free ABEI in these conditions were examined. We suggest also that the oxidative deamination of taurine chloramine leads to the formation of the various carbonyl derivatives and their formation is accelerated in the presence of H(2)O(2), especially in less alkaline solutions (pH 11). Moreover, the formation of enol forms of aldehydes in assay buffers was observed. The yield of the phenoxy radical mediators of ABEI oxidation and the pH-dependent H(2)O(2):HO(2) ratio seems to be decisive for the overall CL in the system examined. The main advantage of this method is that CL does not need precise timing of measurements and assays can be performed over a long period of time (hours) using a plate luminometer.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1522-7235
pubmed:author
pubmed:copyrightInfo
Copyright 1999 John Wiley & Sons, Ltd.
pubmed:issnType
Print
pubmed:volume
14
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
139-45
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:articleTitle
Chemiluminescence of ABEI-labelled IgG triggered by the N-chloramine-H(2)O(2)-p-iodophenol system.
pubmed:affiliation
Jagiellonian University College of Medicine, Institute of Medical Biochemistry, 31-034 Krakow, ul Kopernika 7, Poland.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't