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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1999-9-7
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pubmed:abstractText |
Three of the four known mouse collectin genes have been mapped to chromosome 14. To further characterize the spatial relationship of these genes, a bacterial artificial chromosome (BAC) library of mouse chromosome 14 was screened using mouse surfactant protein (SP)-A and -D complementary DNAs (cDNAs). One large clone hybridized to both SP-A and SP-D cDNAs and was found by polymerase chain reaction (PCR) to contain sequences from one of the mouse mannose-binding lectin genes (Mbl1). We used Southern mapping and subcloning of overlapping restriction fragments to characterize the gene locus. Mapping was confirmed by fluorescent in situ hybridization of fiber-stretched BAC DNA and by Southern hybridization of restriction endonuclease-digested and PCR-amplified genomic DNA. We found that the SP-A, Mbl1, and SP-D genes reside contiguously within a 55-kb region. The SP-A and Mbl1 genes are in the same 5' to 3' orientation and 16 kb apart. The SP-D gene is in the opposite orientation to the two other collectin genes, 13 kb away from the 3' end of the Mbl1 gene. The mouse SP-D gene had not previously been characterized. We found its size (13 kb) and organization to be similar to that of human SP-D. Exon I is untranslated. The second exon is a hybrid exon that contains signal for initiation of translation, signal peptide, N-terminal domain, and the first seven collagen triplets of the collagen-like domain of the protein. Four short exons (III through VI) encode the collagen-like domain of the protein, and exons VII and VIII the linking and the carbohydrate-recognition domains, respectively.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Collectins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Mannose-Binding Lectin,
http://linkedlifedata.com/resource/pubmed/chemical/Mannose-Binding Lectins,
http://linkedlifedata.com/resource/pubmed/chemical/Proteolipids,
http://linkedlifedata.com/resource/pubmed/chemical/Pulmonary Surfactant-Associated...,
http://linkedlifedata.com/resource/pubmed/chemical/Pulmonary Surfactant-Associated...,
http://linkedlifedata.com/resource/pubmed/chemical/Pulmonary Surfactant-Associated...,
http://linkedlifedata.com/resource/pubmed/chemical/Pulmonary Surfactants,
http://linkedlifedata.com/resource/pubmed/chemical/mannose binding protein A
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
1044-1549
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
21
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
193-9
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:10423401-Animals,
pubmed-meshheading:10423401-Base Sequence,
pubmed-meshheading:10423401-Blotting, Southern,
pubmed-meshheading:10423401-Carrier Proteins,
pubmed-meshheading:10423401-Chromosome Mapping,
pubmed-meshheading:10423401-Chromosomes, Bacterial,
pubmed-meshheading:10423401-Cloning, Molecular,
pubmed-meshheading:10423401-Collectins,
pubmed-meshheading:10423401-DNA, Complementary,
pubmed-meshheading:10423401-Exons,
pubmed-meshheading:10423401-Genomic Library,
pubmed-meshheading:10423401-Glycoproteins,
pubmed-meshheading:10423401-In Situ Hybridization, Fluorescence,
pubmed-meshheading:10423401-Introns,
pubmed-meshheading:10423401-Mannose-Binding Lectin,
pubmed-meshheading:10423401-Mannose-Binding Lectins,
pubmed-meshheading:10423401-Mice,
pubmed-meshheading:10423401-Models, Genetic,
pubmed-meshheading:10423401-Molecular Sequence Data,
pubmed-meshheading:10423401-Proteolipids,
pubmed-meshheading:10423401-Pulmonary Surfactant-Associated Protein A,
pubmed-meshheading:10423401-Pulmonary Surfactant-Associated Protein D,
pubmed-meshheading:10423401-Pulmonary Surfactant-Associated Proteins,
pubmed-meshheading:10423401-Pulmonary Surfactants
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pubmed:year |
1999
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pubmed:articleTitle |
Characterization of the mouse collectin gene locus.
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pubmed:affiliation |
Cardiovascular Research Institute and Department of Pediatrics, University of California San Francisco, San Francisco, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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