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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1999-8-13
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pubmed:abstractText |
Steroid hormones are small lipophilic molecules that control a wide range of responses in both the developing and adult organism. The actions of these molecules are mediated by soluble receptor proteins that function as hormone-activated transcription factors. The first steroid receptors were expressed in the yeast Saccharomyces cerevisiae over 10 years ago, and to date virtually all the classical steroid receptors, together with a number of non-steroid members of the nuclear receptor superfamily, have been expressed in yeast. The ability to reconstitute steroid receptor signalling in yeast cells by co-expression of the receptor protein and a reporter gene driven by the appropriate hormone response element has presented researchers with a powerful model system for investigating receptor action. In this review, the use of yeast-based steroid receptor transactivation assays to investigate the roles of molecular chaperones, the mechanisms of DNA binding and gene activation, and the functional properties of hormone mimics will be discussed.
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Chromatin,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Fungal,
http://linkedlifedata.com/resource/pubmed/chemical/Fungal Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/HSP40 Heat-Shock Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/HSP70 Heat-Shock Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/HSP82 protein, S cerevisiae,
http://linkedlifedata.com/resource/pubmed/chemical/HSP90 Heat-Shock Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Heat-Shock Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Ligands,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Steroid,
http://linkedlifedata.com/resource/pubmed/chemical/Saccharomyces cerevisiae Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/YDJ1 protein, S cerevisiae
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0378-1097
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
176
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1-9
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pubmed:dateRevised |
2009-5-21
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pubmed:meshHeading |
pubmed-meshheading:10418126-Amino Acid Sequence,
pubmed-meshheading:10418126-Chromatin,
pubmed-meshheading:10418126-DNA, Fungal,
pubmed-meshheading:10418126-Fungal Proteins,
pubmed-meshheading:10418126-Genes, Fungal,
pubmed-meshheading:10418126-HSP40 Heat-Shock Proteins,
pubmed-meshheading:10418126-HSP70 Heat-Shock Proteins,
pubmed-meshheading:10418126-HSP90 Heat-Shock Proteins,
pubmed-meshheading:10418126-Heat-Shock Proteins,
pubmed-meshheading:10418126-Ligands,
pubmed-meshheading:10418126-Molecular Sequence Data,
pubmed-meshheading:10418126-Receptors, Steroid,
pubmed-meshheading:10418126-Saccharomyces cerevisiae,
pubmed-meshheading:10418126-Saccharomyces cerevisiae Proteins,
pubmed-meshheading:10418126-Transcriptional Activation
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pubmed:year |
1999
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pubmed:articleTitle |
Investigation of steroid receptor function in the budding yeast Saccharomyces cerevisiae.
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pubmed:affiliation |
Department of Molecular and Cell Biology, University of Aberdeen, Foresterhill, UK. iain.mcewan@abdn.ac.uk
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pubmed:publicationType |
Journal Article,
Review,
Research Support, Non-U.S. Gov't
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