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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1999-8-13
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pubmed:abstractText |
We developed a universal recombinant bispecific molecule (BiMol) that is capable of redirecting cytotoxic T cells to tumor cells via tagged anti-tumor ligands such as antibody fragments or cytokines. A recombinant bispecific diabody with binding specificities for the CD3 molecule on T cells as well as for the hapten nitrophenyl (NIP) was produced. This bispecific molecule is capable of redirecting cytotoxic T cells to kill a series of malignant cells, including B cell lymphoma, Hodgkin's lymphoma, and colon carcinoma via NIP-conjugated ligands to tumor-associated antigens. Cytotoxic activity of the diabody was found to be comparable to tetradoma-derived bispecific antibodies with similar specificities. Our findings demonstrate that universal CD3xanti-NIP diabodies could be used for T cell based cellular immunotherapy in a variety of human malignancies. Additionally, these bispecific molecules allow fast and economic testing of tumor-associated antigens on malignant cells for their potential use as immunotherapeutic target structures if corresponding hapten-conjugated antibodies or ligands are available.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/4-hydroxy-5-nitrophenyl acetic acid,
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Bispecific,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD3,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Neoplasm,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Haptens,
http://linkedlifedata.com/resource/pubmed/chemical/Nitrophenols,
http://linkedlifedata.com/resource/pubmed/chemical/Phenylacetates
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0020-7136
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pubmed:author |
pubmed-author:BohlesMM,
pubmed-author:DiehlVV,
pubmed-author:FitzgeraldK JKJ,
pubmed-author:FleischmannBB,
pubmed-author:HeschelerJJ,
pubmed-author:HolligerPP,
pubmed-author:HoogenboomH RHR,
pubmed-author:JohnsonK SKS,
pubmed-author:KlockJJ,
pubmed-author:ManzkeOO,
pubmed-author:QinghuaLL,
pubmed-author:SpanMM
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pubmed:copyrightInfo |
Copyright 1999 Wiley-Liss, Inc.
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pubmed:issnType |
Print
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pubmed:day |
27
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pubmed:volume |
82
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
700-8
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:10417768-Antibodies, Bispecific,
pubmed-meshheading:10417768-Antigens, CD3,
pubmed-meshheading:10417768-Antigens, Neoplasm,
pubmed-meshheading:10417768-Calcium,
pubmed-meshheading:10417768-Cell Division,
pubmed-meshheading:10417768-Haptens,
pubmed-meshheading:10417768-Humans,
pubmed-meshheading:10417768-Hybridomas,
pubmed-meshheading:10417768-Jurkat Cells,
pubmed-meshheading:10417768-Neoplasms,
pubmed-meshheading:10417768-Nitrophenols,
pubmed-meshheading:10417768-Phenylacetates,
pubmed-meshheading:10417768-T-Lymphocytes, Cytotoxic,
pubmed-meshheading:10417768-Tumor Cells, Cultured
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pubmed:year |
1999
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pubmed:articleTitle |
CD3X anti-nitrophenyl bispecific diabodies: universal immunotherapeutic tools for retargeting T cells to tumors.
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pubmed:affiliation |
Department I of Internal Medicine, University of Cologne, Cologne, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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