Source:http://linkedlifedata.com/resource/pubmed/id/10415042
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1999-8-12
|
| pubmed:abstractText |
To analyze the effect of IL-10 overexpressed by APCs as observed in some SCID patients, we have expressed the human IL-10 cDNA under the control of the murine MHC class II promoter in transgenic mice. Similar to SCID patients, these mice presented a defect in T cell maturation characterized by a rapid thymic aplasia that started after birth. The blockage in T cell maturation was strictly restricted to TCR-alpha beta T cells as the absolute number of thymic dendritic, TCR-gamma delta and NK1.1 T cells were equivalent to control littermates. Crossing IL-10 transgenic mice with TCR transgenic mice or treatment with staphylococcal enterotoxin B showed that the defect was not related to the impairment of positive or negative selection. However, repopulating of IL-10 transgenic mouse-fetal thymic organ culture with different stages of triple negative T cells isolated from control mice showed that the blockage occurred specifically at the pre-T cell stage and was reverted by treatment with blocking anti-IL-10 mAbs. These results demonstrate that IL-10 regulates T cell maturation and that dysregulation of IL-10 expression can lead to severe T cell immunodeficiency.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Aug
|
| pubmed:issn |
0022-1767
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
163
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1420-7
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:10415042-Animals,
pubmed-meshheading:10415042-Animals, Newborn,
pubmed-meshheading:10415042-CD4-Positive T-Lymphocytes,
pubmed-meshheading:10415042-CD8-Positive T-Lymphocytes,
pubmed-meshheading:10415042-Cell Differentiation,
pubmed-meshheading:10415042-Dendritic Cells,
pubmed-meshheading:10415042-Fetus,
pubmed-meshheading:10415042-Humans,
pubmed-meshheading:10415042-Interleukin-10,
pubmed-meshheading:10415042-Killer Cells, Natural,
pubmed-meshheading:10415042-Male,
pubmed-meshheading:10415042-Mice,
pubmed-meshheading:10415042-Mice, Inbred BALB C,
pubmed-meshheading:10415042-Mice, Transgenic,
pubmed-meshheading:10415042-Organ Culture Techniques,
pubmed-meshheading:10415042-Receptors, Antigen, T-Cell, gamma-delta,
pubmed-meshheading:10415042-Severe Combined Immunodeficiency,
pubmed-meshheading:10415042-Stem Cells,
pubmed-meshheading:10415042-Stromal Cells,
pubmed-meshheading:10415042-T-Lymphocytes,
pubmed-meshheading:10415042-Thymus Gland
|
| pubmed:year |
1999
|
| pubmed:articleTitle |
IL-10 transgenic mice present a defect in T cell development reminiscent of SCID patients.
|
| pubmed:affiliation |
DNAX Research Institute, Palo Alto, CA 94304, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|