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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1999-8-12
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pubmed:abstractText |
Whether the sequelae of signals generated through CD28 either directly or in circumstances of costimulation require proximal events mediated by p56lck remains contentious. We demonstrate that CD4-, but not CD4+ clonal variants respond to CD28-specific mAb with both early and late indicators of activation. Forced expression of A418/A420-mutated CD4 or wild-type CD4 in the CD4- variant recapitulated the CD28-mediated responses of the CD4- and CD4+ variants, respectively. The implicated involvement of non-CD4-associated Lck is formally demonstrated by overexpressing S20/S23 Lck or wild-type Lck in CD4+ variants. The former, but not latter, rescues direct CD28 signaling, and supports costimulation. The results demonstrate that constitutive levels of non-CD4-associated Lck functionally limit CD28-mediated signaling.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD28,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD4,
http://linkedlifedata.com/resource/pubmed/chemical/Cysteine,
http://linkedlifedata.com/resource/pubmed/chemical/Lymphocyte Specific Protein...,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, T-Cell...
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0022-1767
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
163
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1334-41
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:10415032-Animals,
pubmed-meshheading:10415032-Antibodies, Monoclonal,
pubmed-meshheading:10415032-Antigens, CD28,
pubmed-meshheading:10415032-Antigens, CD4,
pubmed-meshheading:10415032-Cell Division,
pubmed-meshheading:10415032-Cell Line,
pubmed-meshheading:10415032-Clone Cells,
pubmed-meshheading:10415032-Cysteine,
pubmed-meshheading:10415032-Genetic Vectors,
pubmed-meshheading:10415032-Lymphocyte Activation,
pubmed-meshheading:10415032-Lymphocyte Specific Protein Tyrosine Kinase p56(lck),
pubmed-meshheading:10415032-Male,
pubmed-meshheading:10415032-Mice,
pubmed-meshheading:10415032-Mice, Inbred C57BL,
pubmed-meshheading:10415032-Mice, Mutant Strains,
pubmed-meshheading:10415032-Receptors, Antigen, T-Cell, alpha-beta,
pubmed-meshheading:10415032-Retroviridae,
pubmed-meshheading:10415032-Signal Transduction,
pubmed-meshheading:10415032-T-Lymphocyte Subsets
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pubmed:year |
1999
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pubmed:articleTitle |
TCR alpha beta-independent CD28 signaling and costimulation require non-CD4-associated Lck.
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pubmed:affiliation |
Department of Immunology, University of Toronto, Arthritis and Immune Disorder Research Centre, Ontario, Canada.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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