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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
15
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pubmed:dateCreated |
1999-8-16
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pubmed:databankReference |
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pubmed:abstractText |
The POU-IV or Brn-3 class of transcription factors exhibit conserved structure, DNA-binding properties, and expression in specific subclasses of neurons across widely diverged species. In the mouse CNS, Brn-3.0 expression characterizes specific neurons from neurogenesis through the life of the cell. This irreversible activation of expression suggests positive autoregulation. To search for cis-acting elements that could mediate autoregulation we used a novel method, complex stability screening, which we applied to rapidly identify functional Brn-3.0 recognition sites within a large genomic region encompassing the mouse brn-3.0 locus. This method is based on the observation that the kinetic stability of Brn-3.0 complexes with specific DNA sequences, as measured by their dissociation half-lives, is highly correlated with the ability of those sequences to mediate transcriptional activation by Brn-3.0. The principal Brn-3.0 autoregulatory region lies approximately 5 kb upstream from the Brn-3.0 transcription start site and contains multiple Brn-3.0-binding sites that strongly resemble the optimal binding site for this protein class. This region also mediates transactivation by the closely related protein Brn-3.2, suggesting a regulatory cascade of POU proteins in specific neurons in which Brn-3.2 expression precedes Brn-3.0.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Homeodomain Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Pou4f1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Pou4f2 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factor Brn-3,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factor Brn-3A,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factor Brn-3B,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0270-6474
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
19
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
6549-58
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:10414983-Aging,
pubmed-meshheading:10414983-Animals,
pubmed-meshheading:10414983-Chromosome Mapping,
pubmed-meshheading:10414983-DNA,
pubmed-meshheading:10414983-DNA-Binding Proteins,
pubmed-meshheading:10414983-Drug Stability,
pubmed-meshheading:10414983-Embryo, Mammalian,
pubmed-meshheading:10414983-Embryonic and Fetal Development,
pubmed-meshheading:10414983-Genetic Techniques,
pubmed-meshheading:10414983-Genome,
pubmed-meshheading:10414983-Homeodomain Proteins,
pubmed-meshheading:10414983-Homeostasis,
pubmed-meshheading:10414983-Immunohistochemistry,
pubmed-meshheading:10414983-Mice,
pubmed-meshheading:10414983-Time Factors,
pubmed-meshheading:10414983-Transcription Factor Brn-3,
pubmed-meshheading:10414983-Transcription Factor Brn-3A,
pubmed-meshheading:10414983-Transcription Factor Brn-3B,
pubmed-meshheading:10414983-Transcription Factors,
pubmed-meshheading:10414983-Transcriptional Activation
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pubmed:year |
1999
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pubmed:articleTitle |
Autoregulatory sequences are revealed by complex stability screening of the mouse brn-3.0 locus.
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pubmed:affiliation |
Department of Psychiatry, University of California San Diego, La Jolla, California 92093-0603, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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