Linked Life Data

10414780

Source:http://linkedlifedata.com/resource/pubmed/id/10414780

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-3
pubmed:dateCreated
1999-7-29
pubmed:abstractText
A direct continuous spectrophotometric assay to measure pyrethroid-cleaving enzymes in human serum was developed using cis- and trans-alpha-naphthyl-(2,2-dichlorovinyl)-2,2-dimethylcyclopropane carboxylate (cis- and trans-naphthyl-Cl2CA). These substrates show a structure very similar to the pyrethroids most often used (e.g. permethrin, cyfluthrin). The method is based on an increase in absorbance at 321 nm which occurs with the hydrolysis of the alpha-naphthyl esters to alpha-naphthol. The assay was optimised regarding type of buffer, pH and substrate concentrations, it was linear for at least 10 min at 37 degrees C. These esterases were completely inhibited by bis-(4-nitrophenylphosphate), a specific carboxyesterase inhibitor. They displayed a great individual variability in human serum, activities were between less than 40 and 1000 U/l for cis-naphthyl-Cl2CA, between less than 40 and 2000 U/l for trans-naphthyl-Cl2CA, respectively. However, a correlation of enzyme activity to sex or age could not be observed. Furthermore, the activity of pyrethroid-cleaving esterases did not correspond to the activities of acylesterase, arylesterase, acetylcholinesterase or butyrylcholinesterase.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0378-4274
pubmed:author
pubmed:issnType
Print
pubmed:day
30
pubmed:volume
107
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
49-53
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1999
pubmed:articleTitle
A spectrophotometric assay for pyrethroid-cleaving enzymes in human serum.
pubmed:affiliation
Carl von Ossietzky Universität Oldenburg, Fachbereich Chemie, Germany. butte@chemie.uni-oldenburg.de
pubmed:publicationType
Journal Article