Linked Life Data

10413625

Source:http://linkedlifedata.com/resource/pubmed/id/10413625

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1999-8-25
pubmed:databankReference
pubmed:abstractText
Chloroplast-expressed glutamine synthetase (ncpGS), a nuclear-encoded gene containing several introns, is introduced as a tool for phylogenetic studies at lower taxonomic levels. This gene is a member of a multigene family, but it diverged long ago from the cytosolic-expressed members of the family and appears to be single copy in the majority of taxa examined to date. The conservation of both coding sequence and position of introns has allowed the design of primers for use in a broad range of dicot taxa to amplify and sequence a region of ncpGS that contains four introns. The utility of this region in phylogenetic studies of congeneric species is illustrated by an example using eight Oxalis species. The four introns in these taxa are typical in size (76 to 136 bp), base composition (high T content), and structure (e.g., sequence of splice sites and putative branch points) for plant internal introns. Levels of variation among these ncpGS sequences compare favorably with those of the internal transcribed spacer of nuclear ribosomal DNA (ITS) from the same taxa, and results of phylogenetic analysis of ncpGS data are generally congruent with previous results using ITS.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
1055-7903
pubmed:author
pubmed:copyrightInfo
Copyright 1999 Academic Press.
pubmed:issnType
Print
pubmed:volume
12
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
310-9
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:10413625-Base Sequence, pubmed-meshheading:10413625-Cell Nucleus, pubmed-meshheading:10413625-Chloroplasts, pubmed-meshheading:10413625-DNA, Plant, pubmed-meshheading:10413625-DNA, Ribosomal, pubmed-meshheading:10413625-DNA Primers, pubmed-meshheading:10413625-Gene Expression Regulation, Enzymologic, pubmed-meshheading:10413625-Gene Expression Regulation, Plant, pubmed-meshheading:10413625-Genetic Variation, pubmed-meshheading:10413625-Glutamate-Ammonia Ligase, pubmed-meshheading:10413625-Molecular Sequence Data, pubmed-meshheading:10413625-Mutation, pubmed-meshheading:10413625-Phylogeny, pubmed-meshheading:10413625-Plants, pubmed-meshheading:10413625-Sequence Alignment, pubmed-meshheading:10413625-Sequence Analysis, DNA, pubmed-meshheading:10413625-Sequence Homology, Nucleic Acid
pubmed:year
1999
pubmed:articleTitle
Chloroplast-expressed glutamine synthetase (ncpGS): potential utility for phylogenetic studies with an example from Oxalis (Oxalidaceae).
pubmed:affiliation
L. H. Bailey Hortorium, Cornell University, Ithaca, New York 14853, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't