Linked Life Data

10413052

Source:http://linkedlifedata.com/resource/pubmed/id/10413052

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
1999-10-5
pubmed:databankReference
pubmed:abstractText
A fragment of a cut-1 like gene from the filarial nematode Brugia pahangi (designated Bp-cut-1) was isolated by PCR from genomic DNA. The sequence was used to design primers for use in RT-PCR and resulted in the isolation of a cDNA fragment from larvae in the process of the L3-L4 moult. Screening of a B. malayi genomic library identified a single clone, Bm-cut-1. Using primers designed from the Brugia sequences, semi-quantitative RT-PCR was carried out on 11 different life cycle stages chosen to cover periods around the moult and inter-moult periods. This analysis demonstrated that the cut-1 mRNA was most abundant preceding the moult, consistent with its function as a cuticular protein. Immuno-gold electron microscopy using an affinity purified antiserum raised to the highly conserved region of Ascaris CUT-1 confirmed that the protein was restricted to a tight band in the median layer of the cuticle. Despite the fact that no transcripts could be detected in mature adult worms by RT-PCR, immuno-gold microscopy revealed staining of the microfilarial cuticle within the uterus of the adult female worm, suggesting that other cut-1-like genes are present in Brugia.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0166-6851
pubmed:author
pubmed:issnType
Print
pubmed:day
25
pubmed:volume
101
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
173-83
pubmed:dateRevised
2010-8-25
pubmed:meshHeading
pubmed-meshheading:10413052-Amino Acid Sequence, pubmed-meshheading:10413052-Animals, pubmed-meshheading:10413052-Brugia malayi, pubmed-meshheading:10413052-Brugia pahangi, pubmed-meshheading:10413052-Caenorhabditis elegans Proteins, pubmed-meshheading:10413052-Cloning, Molecular, pubmed-meshheading:10413052-DNA, Complementary, pubmed-meshheading:10413052-Female, pubmed-meshheading:10413052-Gene Library, pubmed-meshheading:10413052-Genes, Helminth, pubmed-meshheading:10413052-Helminth Proteins, pubmed-meshheading:10413052-Immunohistochemistry, pubmed-meshheading:10413052-Male, pubmed-meshheading:10413052-Microscopy, Electron, pubmed-meshheading:10413052-Molecular Sequence Data, pubmed-meshheading:10413052-RNA, Messenger, pubmed-meshheading:10413052-Reverse Transcriptase Polymerase Chain Reaction
pubmed:year
1999
pubmed:articleTitle
cut-1-like genes are present in the filarial nematodes, Brugia pahangi and Brugia malayi, and, as in other nematodes, code for components of the cuticle.
pubmed:affiliation
Department of Veterinary Parasitology, University of Glasgow, UK.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't