| pubmed-article:10411920 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10411920 | lifeskim:mentions | umls-concept:C0017296 | lld:lifeskim |
| pubmed-article:10411920 | lifeskim:mentions | umls-concept:C0021756 | lld:lifeskim |
| pubmed-article:10411920 | lifeskim:mentions | umls-concept:C0123759 | lld:lifeskim |
| pubmed-article:10411920 | lifeskim:mentions | umls-concept:C1318468 | lld:lifeskim |
| pubmed-article:10411920 | lifeskim:mentions | umls-concept:C0205263 | lld:lifeskim |
| pubmed-article:10411920 | pubmed:issue | 15 | lld:pubmed |
| pubmed-article:10411920 | pubmed:dateCreated | 1999-8-23 | lld:pubmed |
| pubmed-article:10411920 | pubmed:abstractText | Induction, maintenance, and amplification of tumor-protective immunity after cytokine gene therapy is essential for the clinical success of immunotherapeutic approaches. We investigated whether this could be achieved by single-chain IL-12 (scIL-12) gene therapy followed by tumor-targeted IL-2 using a fusion protein containing a tumor-specific recombinant anti-ganglioside GD(2) antibody and IL-2 (ch14.18-IL-2) in a poorly immunogenic murine neuroblastoma model. Herein, we demonstrate the absence of liver and bone marrow metastases after a lethal challenge with NXS2 wild-type cells only in mice (five of six animals) vaccinated with scIL-12-producing NXS2 cells and given a booster injection of low-dose ch14.18-IL-2 fusion protein. This tumor-protective immunity was effective 3 months after initial vaccination, in contrast to control animals treated with a nonspecific fusion protein or an equivalent mixture of antibody and IL-2. Only vaccinated mice receiving the tumor-specific ch14.18-IL-2 fusion protein revealed a reactivation of CD8(+) T cells and subsequent MHC class I-restricted tumor target cell lysis in vitro. The sequential increase in the usage of TCR chains Vbeta11 and -13 in mouse CD8(+) T cells after vaccination and amplification with ch14.18-IL-2 suggests that the initial polyclonal CD8(+) T cell response is effectively boosted by targeted IL-2. In conclusion, we demonstrate that a successful boost of a partially protective memory T cell immune response that is induced by scIL-12 gene therapy could be generated by tumor-specific targeting of IL-2 with a ch14.18-IL-2 fusion protein. This approach could increase success rates of clinical cancer vaccine trials. | lld:pubmed |
| pubmed-article:10411920 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10411920 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10411920 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10411920 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10411920 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10411920 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10411920 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10411920 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10411920 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10411920 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10411920 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10411920 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10411920 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10411920 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10411920 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10411920 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10411920 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10411920 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10411920 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10411920 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10411920 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10411920 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10411920 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10411920 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10411920 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10411920 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10411920 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10411920 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10411920 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10411920 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:10411920 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10411920 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10411920 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10411920 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10411920 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10411920 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10411920 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10411920 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10411920 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10411920 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10411920 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:10411920 | pubmed:issn | 0027-8424 | lld:pubmed |
| pubmed-article:10411920 | pubmed:author | pubmed-author:Theofilopoulo... | lld:pubmed |
| pubmed-article:10411920 | pubmed:author | pubmed-author:ReisfeldR ARA | lld:pubmed |
| pubmed-article:10411920 | pubmed:author | pubmed-author:DuncanS RSR | lld:pubmed |
| pubmed-article:10411920 | pubmed:author | pubmed-author:GilliesS DSD | lld:pubmed |
| pubmed-article:10411920 | pubmed:author | pubmed-author:LodeH NHN | lld:pubmed |
| pubmed-article:10411920 | pubmed:author | pubmed-author:XiangRR | lld:pubmed |
| pubmed-article:10411920 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10411920 | pubmed:day | 20 | lld:pubmed |
| pubmed-article:10411920 | pubmed:volume | 96 | lld:pubmed |
| pubmed-article:10411920 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10411920 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10411920 | pubmed:pagination | 8591-6 | lld:pubmed |
| pubmed-article:10411920 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
| pubmed-article:10411920 | pubmed:meshHeading | pubmed-meshheading:10411920... | lld:pubmed |
| pubmed-article:10411920 | pubmed:meshHeading | pubmed-meshheading:10411920... | lld:pubmed |
| pubmed-article:10411920 | pubmed:meshHeading | pubmed-meshheading:10411920... | lld:pubmed |
| pubmed-article:10411920 | pubmed:meshHeading | pubmed-meshheading:10411920... | lld:pubmed |
| pubmed-article:10411920 | pubmed:meshHeading | pubmed-meshheading:10411920... | lld:pubmed |
| pubmed-article:10411920 | pubmed:meshHeading | pubmed-meshheading:10411920... | lld:pubmed |
| pubmed-article:10411920 | pubmed:meshHeading | pubmed-meshheading:10411920... | lld:pubmed |
| pubmed-article:10411920 | pubmed:meshHeading | pubmed-meshheading:10411920... | lld:pubmed |
| pubmed-article:10411920 | pubmed:meshHeading | pubmed-meshheading:10411920... | lld:pubmed |
| pubmed-article:10411920 | pubmed:meshHeading | pubmed-meshheading:10411920... | lld:pubmed |
| pubmed-article:10411920 | pubmed:meshHeading | pubmed-meshheading:10411920... | lld:pubmed |
| pubmed-article:10411920 | pubmed:meshHeading | pubmed-meshheading:10411920... | lld:pubmed |
| pubmed-article:10411920 | pubmed:meshHeading | pubmed-meshheading:10411920... | lld:pubmed |
| pubmed-article:10411920 | pubmed:meshHeading | pubmed-meshheading:10411920... | lld:pubmed |
| pubmed-article:10411920 | pubmed:meshHeading | pubmed-meshheading:10411920... | lld:pubmed |
| pubmed-article:10411920 | pubmed:meshHeading | pubmed-meshheading:10411920... | lld:pubmed |
| pubmed-article:10411920 | pubmed:meshHeading | pubmed-meshheading:10411920... | lld:pubmed |
| pubmed-article:10411920 | pubmed:meshHeading | pubmed-meshheading:10411920... | lld:pubmed |
| pubmed-article:10411920 | pubmed:year | 1999 | lld:pubmed |
| pubmed-article:10411920 | pubmed:articleTitle | Tumor-targeted IL-2 amplifies T cell-mediated immune response induced by gene therapy with single-chain IL-12. | lld:pubmed |
| pubmed-article:10411920 | pubmed:affiliation | The Scripps Research Institute, Department of Immunology, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA. | lld:pubmed |
| pubmed-article:10411920 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:10411920 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:10411920 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10411920 | lld:pubmed |
