Linked Life Data

10411727

Source:http://linkedlifedata.com/resource/pubmed/id/10411727

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1999-9-7
pubmed:databankReference
pubmed:abstractText
The extracytoplasmic function (ECF) sigma factor, sigmaE, is required for normal cell wall integrity in Streptomyces coelicolor. We have investigated the regulation of sigmaE through a transcriptional and mutational analysis of sigE and the surrounding genes. Nucleotide sequencing identified three genes located downstream of sigE; orf202, cseB and cseC (cse, control of sigE ). cseB and cseC encode a putative response regulator and a putative transmembrane sensor histidine protein kinase respectively. Although most sigE transcription appeared to be monocistronic, sigE was also transcribed as part of a larger operon, including at least orf202. sigE null mutants are sensitive to cell wall lytic enzymes, have an altered peptidoglycan muropeptide profile, and on medium deficient in Mg2+ they overproduce actinorhodin, sporulate poorly and form crenellated colonies. A constructed cseB null mutant appeared to have the same phenotype as a sigE null mutant, which was accounted for by the observed absolute dependence of the sigE promoter on cseB. It is likely that the major role of cseB is to regulate sigE transcription because expression of sigE alone from a heterologous promoter suppressed the cseB mutation. Mg2+ suppresses the CseB/SigE phenotype, probably by stabilizing the cell envelope, and sigE transcript levels were consistently higher in Mg2+-deficient cultures than in high Mg2+-grown cultures. We propose a model in which the CseB/CseC two-component system modulates activity of the sigE promoter in response to signals from the cell envelope.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0950-382X
pubmed:author
pubmed:issnType
Print
pubmed:volume
33
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
97-107
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:10411727-Amino Acid Sequence, pubmed-meshheading:10411727-Bacterial Proteins, pubmed-meshheading:10411727-Base Sequence, pubmed-meshheading:10411727-Cell Wall, pubmed-meshheading:10411727-Gene Expression Regulation, Bacterial, pubmed-meshheading:10411727-Genes, Bacterial, pubmed-meshheading:10411727-Magnesium, pubmed-meshheading:10411727-Molecular Sequence Data, pubmed-meshheading:10411727-Operon, pubmed-meshheading:10411727-Promoter Regions, Genetic, pubmed-meshheading:10411727-Protein Kinases, pubmed-meshheading:10411727-Recombinant Fusion Proteins, pubmed-meshheading:10411727-Sequence Alignment, pubmed-meshheading:10411727-Sequence Homology, Amino Acid, pubmed-meshheading:10411727-Sigma Factor, pubmed-meshheading:10411727-Signal Transduction, pubmed-meshheading:10411727-Streptomyces, pubmed-meshheading:10411727-Transcription, Genetic, pubmed-meshheading:10411727-Transcription Factors
pubmed:year
1999
pubmed:articleTitle
A putative two-component signal transduction system regulates sigmaE, a sigma factor required for normal cell wall integrity in Streptomyces coelicolor A3(2).
pubmed:affiliation
John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, UK. pagetm@bbsrc.ac.uk
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't