Linked Life Data

10408963

Source:http://linkedlifedata.com/resource/pubmed/id/10408963

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
1999-7-12
pubmed:abstractText
Canine distemper virus (CDV) infection in dogs is commonly associated with demyelinating leukoencephalitis (DL). Although the mechanism of primary demyelination in distemper remains undetermined recent studies showed a direct virus-induced cytolysis in early non-inflammatory and immune-mediated mechanisms in inflammatory lesions. To further investigate the pathogenesis of this morbillivirus-induced demyelination the expression of a variety of cytokine mRNA species (interleukin (IL)-1beta, IL-2, IL-6, IL-10, IL-12, tumor necrosis factor (TNF)-alpha, transforming growth factor (TGF)-beta1, and interferon (IFN)-gamma in cerebrospinal fluid cells of 12 dogs with CDV encephalitis was investigated employing reverse transcription-polymerase chain reaction (RT-PCR) and these findings were correlated to the type of CNS lesions. Neuropathology revealed the whole spectrum of distemper DL lesions from acute to chronic alterations, however, most plaques lacked active demyelination. Three control animals were devoid of any cytokine expression, whereas in distemper animals IL-10 transcripts were found in nine dogs with acute and chronic lesions. IL-6, TNF, and TGF mRNA was found in six, four, and three animals, respectively. IL-12 and IFN-gamma, suggestive of a TH1-like dominated immune response, were detected only in one animal with chronic lesions. Summarized, TNF and IL-6, associated with disease exacerbation, and IL-10 and TGF, indicative of remission, were often observed simultaneously in distemper DL and could not be assigned to a specific disease stage. However IL-10 mRNA remained the most frequently detected cytokine indicating a stage of inactivity in most animals investigated.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0165-5728
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
97
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
102-9
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:10408963-Animals, pubmed-meshheading:10408963-Brain, pubmed-meshheading:10408963-DNA Primers, pubmed-meshheading:10408963-Demyelinating Diseases, pubmed-meshheading:10408963-Disease Models, Animal, pubmed-meshheading:10408963-Distemper, pubmed-meshheading:10408963-Distemper Virus, Canine, pubmed-meshheading:10408963-Dogs, pubmed-meshheading:10408963-Encephalitis, Viral, pubmed-meshheading:10408963-Gene Expression, pubmed-meshheading:10408963-Interferon-gamma, pubmed-meshheading:10408963-Interleukin-1, pubmed-meshheading:10408963-Interleukin-10, pubmed-meshheading:10408963-Interleukin-12, pubmed-meshheading:10408963-Interleukin-2, pubmed-meshheading:10408963-Interleukin-6, pubmed-meshheading:10408963-Multiple Sclerosis, pubmed-meshheading:10408963-RNA, Messenger, pubmed-meshheading:10408963-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:10408963-Transforming Growth Factor beta, pubmed-meshheading:10408963-Tumor Necrosis Factor-alpha
pubmed:year
1999
pubmed:articleTitle
Dominating interleukin-10 mRNA expression induction in cerebrospinal fluid cells of dogs with natural canine distemper virus induced demyelinating and non-demyelinating CNS lesions.
pubmed:affiliation
Institut für Veterinär-Pathologie, Justus-Liebig-Universität Giessen, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't