Linked Life Data

10407338

Source:http://linkedlifedata.com/resource/pubmed/id/10407338

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
14
pubmed:dateCreated
1999-9-13
pubmed:abstractText
The four major deoxynucleotides of DNA, and adduct mixtures resulting from separate reactions of 5'-dAMP and 5'-dGMP with benzo[a]pyrene diolepoxide (BPDE), were analyzed by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) after labeling of their phosphate group with an IMI dye. The latter reagent comprises an imidazole functional group attached to a BODIPY (4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene+ ++) fluorophore. Good sensitivity was observed in the detection of the IMI-labeled products by MALDI-MS: 300-500 fmol in the laser spot (1% of the 30-50 pmol sample on the target) gave a signal-to-noise (S/N) of >/=30 from 20-30 superimposed laser shots. The BPDE reaction products, after the IMI labeling, were also subjected to capillary electrophoresis with laser-induced fluorescence detection, which revealed a complex mixture of products. Overall the results encourage the further development of this 'IMI-postlabeling' methodology as an alternative to (32)P-postlabeling for the detection of DNA adducts.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0951-4198
pubmed:author
pubmed:copyrightInfo
Copyright 1999 John Wiley & Sons, Ltd.
pubmed:issnType
Print
pubmed:volume
13
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1454-7
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1999
pubmed:articleTitle
Matrix-assisted laser desorption/ionization mass spectrometry of deoxynucleotides labeled with an IMI dye.
pubmed:affiliation
Department of Pharmaceutical Sciences in the Bouvé College of Pharmacy and Health Professions, Barnett Institute, and Chemistry Department, Northeastern University, Boston, MA 02115, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't