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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1999-8-9
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pubmed:abstractText |
Astrocytes express endothelin-1 (ET-1), ET-3, and their receptors, ET(A) and ET(B). We report here that activated astrocytes in vivo also express endothelin converting enzyme-1 (ECE-1). Higher basal ET-1 concentrations in astrocyte media from ET(B)-deficient (sl/sl) versus wildtype (+/+) rats suggested that altered ECE activity may be related to the absence of ET(B) receptors. Quantification of ECE activity in membranes from sl/sl astrocytes yielded a 50% higher conversion compared to +/+ astrocytes, with indistinguishable ECE-1 mRNA and protein levels. Kinetic analysis of ECE activity revealed similar V(max) values in sl/sl and +/+ astrocytes. Enzyme activity was competitively inhibited by phosphoramidon with K(i) values of 0. 6 and 0.3 microM, respectively. The K(m) value of ECE was 0.5 microM in +/+ and 0.2 microM in sl/sl astrocytes. Two-dimensional focussing of astrocytic ECE-1 uncovered heterogeneity of charge and molecular weight. ECE-1 from sl/sl revealed a glycosylation pattern different from +/+ astrocytes. In conclusion, the ET(B) receptor may, via ECE-1 glycosylation, exert a negative feedback on ECE activity in the astrocytic endothelin system.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Aspartic Acid Endopeptidases,
http://linkedlifedata.com/resource/pubmed/chemical/Culture Media, Conditioned,
http://linkedlifedata.com/resource/pubmed/chemical/Endothelin-1,
http://linkedlifedata.com/resource/pubmed/chemical/Metalloendopeptidases,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, Endothelin B,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Endothelin,
http://linkedlifedata.com/resource/pubmed/chemical/endothelin-converting enzyme
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 1999 Academic Press.
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pubmed:issnType |
Print
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pubmed:day |
22
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pubmed:volume |
261
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
149-55
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:10405338-Animals,
pubmed-meshheading:10405338-Aspartic Acid Endopeptidases,
pubmed-meshheading:10405338-Astrocytes,
pubmed-meshheading:10405338-Blotting, Western,
pubmed-meshheading:10405338-Cell Membrane,
pubmed-meshheading:10405338-Cells, Cultured,
pubmed-meshheading:10405338-Culture Media, Conditioned,
pubmed-meshheading:10405338-Endothelin-1,
pubmed-meshheading:10405338-Genotype,
pubmed-meshheading:10405338-Glycosylation,
pubmed-meshheading:10405338-Immunohistochemistry,
pubmed-meshheading:10405338-Isoelectric Point,
pubmed-meshheading:10405338-Kinetics,
pubmed-meshheading:10405338-Metalloendopeptidases,
pubmed-meshheading:10405338-Protein Processing, Post-Translational,
pubmed-meshheading:10405338-RNA, Messenger,
pubmed-meshheading:10405338-Rats,
pubmed-meshheading:10405338-Rats, Mutant Strains,
pubmed-meshheading:10405338-Rats, Wistar,
pubmed-meshheading:10405338-Receptor, Endothelin B,
pubmed-meshheading:10405338-Receptors, Endothelin,
pubmed-meshheading:10405338-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:10405338-Sequence Deletion
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pubmed:year |
1999
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pubmed:articleTitle |
Endothelin converting enzyme activity in primary rat astrocytes is modulated by endothelin B receptors.
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pubmed:affiliation |
Departments of Neurology, Georg-August-University, Göttingen, Germany. ehrenreich@exmax1.mpiem.gwdg.de
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pubmed:publicationType |
Journal Article
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