Linked Life Data

10404027

Source:http://linkedlifedata.com/resource/pubmed/id/10404027

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
1999-9-28
pubmed:abstractText
We had previously identified the cDNA for a novel protein called osteoblast-specific factor 2 (OSF-2) from an MC3T3-E1 cDNA library using subtraction hybridization and differential screening techniques. Here we describe the localization, regulation, and potential function of this protein. Immunohistochemistry using specific antiserum revealed that in adult mice, the protein is preferentially expressed in periosteum and periodontal ligament, indicating its tissue specificity and a potential role in bone and tooth formation and maintenance of structure. Based on this observation and the fact that other proteins have been called OSF-2, the protein was renamed "periostin." Western blot analysis showed that periostin is a disulfide linked 90 kDa protein secreted by osteoblasts and osteoblast-like cell lines. Nucleotide sequence revealed four periostin transcripts that differ in the length of the C-terminal domain, possibly caused by alternative splicing events. Reverse transcription- polymerase chain reaction analysis revealed that these isoforms are not expressed uniformly but are differentially expressed in various cell lines. Both purified periostin protein and the periostin-Fc recombinant protein supported attachment and spreading of MC3T3-E1 cells, and this effect was impaired by antiperiostin antiserum, suggesting that periostin is involved in cell adhesion. The protein is highly homologous to betaig-h3, a molecule induced by transforming growth factor beta (TGF-beta) that promotes the adhesion and spreading of fibroblasts. Because TGF-beta has dramatic effects on periosteal expansion and the recruitment of osteoblast precursors, this factor was tested for its effects on periostin expression. By Western blot analysis, TGF-beta increased periostin expression in primary osteoblast cells. Together, these data suggest that periostin may play a role in the recruitment and attachment of osteoblast precursors in the periosteum.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0884-0431
pubmed:author
pubmed:issnType
Print
pubmed:volume
14
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1239-49
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:10404027-Amino Acid Sequence, pubmed-meshheading:10404027-Animals, pubmed-meshheading:10404027-Animals, Newborn, pubmed-meshheading:10404027-Blotting, Western, pubmed-meshheading:10404027-Bone Matrix, pubmed-meshheading:10404027-Cell Adhesion Molecules, pubmed-meshheading:10404027-Cell Line, pubmed-meshheading:10404027-Conserved Sequence, pubmed-meshheading:10404027-Extracellular Matrix, pubmed-meshheading:10404027-Gene Expression, pubmed-meshheading:10404027-Mice, pubmed-meshheading:10404027-Molecular Sequence Data, pubmed-meshheading:10404027-Organ Specificity, pubmed-meshheading:10404027-Osteoblasts, pubmed-meshheading:10404027-Periodontal Ligament, pubmed-meshheading:10404027-Periosteum, pubmed-meshheading:10404027-RNA, Messenger, pubmed-meshheading:10404027-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:10404027-Sequence Analysis, DNA, pubmed-meshheading:10404027-Transforming Growth Factor beta
pubmed:year
1999
pubmed:articleTitle
Identification and characterization of a novel protein, periostin, with restricted expression to periosteum and periodontal ligament and increased expression by transforming growth factor beta.
pubmed:affiliation
Department of Life Science, Tokyo Institute of Technology, Yokohama, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't