Linked Life Data

10403768

Source:http://linkedlifedata.com/resource/pubmed/id/10403768

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1999-8-5
pubmed:databankReference
pubmed:abstractText
Articular chondrocytes possess the capacity to express a number of ADAM (A Disintegrin And Metalloproteinase) family members, thereby implicating a role for such proteins in the turnover of cartilage extracellular matrix molecules. Recently, the sequence for the human orthologue of an "aggrecanase" isolated from bovine nasal cartilage has been elucidated, and the recombinant protein product shown to be capable of cleaving aggrecan specifically at the relevant peptide bonds which are hydrolyzed in situ during cartilage degradation. The sequence for the human "aggrecanase" exhibits homology with that of murine ADAMTS-1, an ADAM with thrombospondin type I motifs. In the present study we have identified additional ADAMTS homologues and have examined their mRNA expression profiles in freshly excised human articular cartilage and in human cartilage explant cultures stimulated with IL-1, TNF-alpha, or retinoic acid, agents which enhance "aggrecanase" activity in vitro. Significantly, cartilage exposed to retinoic acid showed a marked increase in the release of "aggrecanase"-generated aggrecan catabolites with no concomitant increase in mRNA levels for any of the ADAMTS homologues investigated. These findings indicate that enhanced "aggrecanase" activity, which may be attributed to known ADAMTS homologues, may be predominantly regulated by post-transcriptional mechanism(s), and may raise the possiblility for the existence of other as yet unidentified "aggrecanase(s)."
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0006-291X
pubmed:author
pubmed:copyrightInfo
Copyright 1999 Academic Press.
pubmed:issnType
Print
pubmed:day
5
pubmed:volume
260
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
318-22
pubmed:dateRevised
2010-7-2
pubmed:meshHeading
pubmed:year
1999
pubmed:articleTitle
Expression of ADAMTS homologues in articular cartilage.
pubmed:affiliation
Cardiff School of Biosciences, Cardiff University, Museum Avenue, PreClinical Buildings, Cardiff, Wales, CF1 3US, United Kingdom. FlanneryCR@Cardiff.ac.uk
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't