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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
7
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pubmed:dateCreated |
1999-7-26
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pubmed:abstractText |
The Brazilian purpuric fever (BPF) clone of Haemophilus influenzae biogroup aegyptius causes a fatal septicaemic disease, resembling fulminant meningococcal sepsis, in children. When isolate F3031 was grown under iron-limiting conditions, the presence of several iron-regulated proteins of 38-110 kDa was revealed by electrophoretic analysis and a Fur homologue was shown by immunoblotting. Dot-blot assays and immunoblotting indicated that BPF cells bound human transferrin and contained transferrin-binding proteins in the outer membrane. However, the binding activity and the biosynthesis of these proteins were detected even under iron-rich conditions. Immunoblot analysis demonstrated the presence of a periplasmic protein related to the ferric iron-binding protein A (FbpA), the major iron-binding protein described in Neisseria spp. However, the FbpA homologue in strain F3031 was constitutively expressed and was smaller than the periplasmic protein detected in H. influenzae type b strain Eagan. The periplasm of strain F3031 also contained a protein related to the Streptococcus parasanguis FimA protein which recently has been shown to be involved in iron acquisition in Yersinia pestis. Although the Eagan and F3031 FimA homologues had a similar mol. wt, of 31 kDa, the expression of the BPF fimA-like gene was not regulated by the iron concentration of the culture medium.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Outer Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Fimbriae Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Hemin,
http://linkedlifedata.com/resource/pubmed/chemical/Iron,
http://linkedlifedata.com/resource/pubmed/chemical/Repressor Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Transferrin,
http://linkedlifedata.com/resource/pubmed/chemical/ferric uptake regulating proteins...,
http://linkedlifedata.com/resource/pubmed/chemical/fibrinogen-binding protein A...,
http://linkedlifedata.com/resource/pubmed/chemical/fimbrillin
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0022-2615
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
48
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
629-36
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:10403413-Bacterial Outer Membrane Proteins,
pubmed-meshheading:10403413-Bacterial Proteins,
pubmed-meshheading:10403413-Blotting, Western,
pubmed-meshheading:10403413-Brazil,
pubmed-meshheading:10403413-Carrier Proteins,
pubmed-meshheading:10403413-Child,
pubmed-meshheading:10403413-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:10403413-Fimbriae Proteins,
pubmed-meshheading:10403413-Gene Expression Regulation, Bacterial,
pubmed-meshheading:10403413-Haemophilus Infections,
pubmed-meshheading:10403413-Haemophilus influenzae,
pubmed-meshheading:10403413-Hemin,
pubmed-meshheading:10403413-Humans,
pubmed-meshheading:10403413-Iron,
pubmed-meshheading:10403413-Molecular Weight,
pubmed-meshheading:10403413-Purpura,
pubmed-meshheading:10403413-Repressor Proteins,
pubmed-meshheading:10403413-Sepsis,
pubmed-meshheading:10403413-Transferrin
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pubmed:year |
1999
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pubmed:articleTitle |
Fur and iron transport proteins in the Brazilian purpuric fever clone of Haemophilus influenzae biogroup aegyptius.
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pubmed:affiliation |
Department of Microbiology, Miami University, Oxford, OH 45056, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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