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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
7
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pubmed:dateCreated |
1999-7-16
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pubmed:abstractText |
The initial identification of GAS6 as a protein expressed in response to growth arrest suggested that it might function as a negative regulator of cell proliferation. Since the transforming activity of the GAS6 receptor (AXL/UFO) was documented, GAS6 might stimulate rather than inhibit proliferation. In order to detect aberrant expression of GAS6 we examined gene expression in 46 cell lines of precursor B-, B- and T-cell origin as well as from Hodgkin's disease and cell lines established from various myeloproliferative disorders. In our study, the expression of GAS6 reveals a constitutive transcriptional activation in 8/46 cases of proliferating cell lines. The GAS6 mRNA expression could be shown in 4/22 cell lines of the lymphoid arm and in 4/17 of the myeloid lineages of the hematopoietic system. No transcripts could be detected in the CD30+ Hodgkin and anaplastic large cell lymphomas (0/7). Interestingly, the steady state mRNA levels showed neglectable GAS6 expression in precursor B and B-cell lines (1/9), but could be detected in terminally differentiated plasma cell lines (4/4). The predominantly GAS6-expressing cell lines of non-lymphoid origin have been established from acute myeloid leukemias of the M4 subtype (3/4). In order to demonstrate evidence for an autocrine regulation of growth in permanent hematopoietic cell lines, we measured the GAS6 expression in cell lines with strong positivity for the AXL/UFO receptor mRNA. Constitutive basal levels of GAS6 mRNA and protein expression could be only detected in 3/23 AXL/UFO expressing cell lines. Although a general mechanism seems most unlikely, further studies are necessary to demonstrate the involvement of GAS6 in single cases of disordered growth or chemotaxis/adhesion of leukemia and lymphomas.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Intercellular Signaling Peptides...,
http://linkedlifedata.com/resource/pubmed/chemical/Neoplasm Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Neoplasm,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor Protein-Tyrosine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/axl receptor tyrosine kinase,
http://linkedlifedata.com/resource/pubmed/chemical/growth arrest-specific protein 6
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0145-2126
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
23
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
643-51
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:10400186-Blotting, Northern,
pubmed-meshheading:10400186-Cell Division,
pubmed-meshheading:10400186-Cell Line,
pubmed-meshheading:10400186-Cloning, Molecular,
pubmed-meshheading:10400186-Gene Expression Regulation, Leukemic,
pubmed-meshheading:10400186-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:10400186-Hematopoietic Stem Cells,
pubmed-meshheading:10400186-Hodgkin Disease,
pubmed-meshheading:10400186-Humans,
pubmed-meshheading:10400186-Intercellular Signaling Peptides and Proteins,
pubmed-meshheading:10400186-Kidney,
pubmed-meshheading:10400186-Leukemia,
pubmed-meshheading:10400186-Lymphocyte Subsets,
pubmed-meshheading:10400186-Lymphoma,
pubmed-meshheading:10400186-Myeloproliferative Disorders,
pubmed-meshheading:10400186-Neoplasm Proteins,
pubmed-meshheading:10400186-Neoplastic Stem Cells,
pubmed-meshheading:10400186-Oncogene Proteins,
pubmed-meshheading:10400186-Protein Biosynthesis,
pubmed-meshheading:10400186-Proteins,
pubmed-meshheading:10400186-Proto-Oncogene Proteins,
pubmed-meshheading:10400186-RNA, Messenger,
pubmed-meshheading:10400186-RNA, Neoplasm,
pubmed-meshheading:10400186-Receptor Protein-Tyrosine Kinases,
pubmed-meshheading:10400186-Recombinant Fusion Proteins,
pubmed-meshheading:10400186-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:10400186-Tumor Cells, Cultured
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pubmed:year |
1999
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pubmed:articleTitle |
Expression of the growth arrest-specific gene 6 (GAS6) in leukemia and lymphoma cell lines.
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pubmed:affiliation |
DSMZ-German Collection of Microorganisms and Cell Cultures, Department of Human and Animal Cell Cultures, Braunschweig. wdi@dsmz.de
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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