Source:http://linkedlifedata.com/resource/pubmed/id/10400065
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1999-10-5
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pubmed:abstractText |
In neocortical neuronal cultures, (S)-AMPA caused neurotoxicity which was concentration-dependent, receptor-mediated, slow and apoptotic in nature. (S)-AMPA (3-600 microM) failed to produce rapid neuronal swelling, but morphological observations and monitoring of viability at 24-72 h revealed 50% cell death consistent with apoptosis. (S)-AMPA induced cell shrinkage, neurite blebbing and nuclear condensation. Cyclothiazide (50 and 100 microM), which blocks AMPA receptor desensitization potentiated excitotoxicity with 75% of neurones undergoing slow death. The AMPA-selective antagonist GYKI 52466 (10-50 microM), attenuated (S)-AMPA-mediated neurotoxicity. DNA condensation, a hallmark of apoptosis, was found by labelling neurones with the DNA binding dye 4,6-diamidino-2-phenylindole HCl (DAPI). Gel electrophoresis revealed DNA fragmentation, which was increased by cyclothiazide and reduced by GYKI 52466 and cycloheximide. Overstimulation of the AMPA receptor produces a novel form of neuronal death, which is apoptotic, very slow in nature, and which could contribute to various neuropathologies.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Anti-Anxiety Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Benzodiazepines,
http://linkedlifedata.com/resource/pubmed/chemical/Benzothiadiazines,
http://linkedlifedata.com/resource/pubmed/chemical/GYKI 52466,
http://linkedlifedata.com/resource/pubmed/chemical/Glutamic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Neuroprotective Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, AMPA,
http://linkedlifedata.com/resource/pubmed/chemical/cyclothiazide
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0304-3940
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
11
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pubmed:volume |
268
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
9-12
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:10400065-Animals,
pubmed-meshheading:10400065-Anti-Anxiety Agents,
pubmed-meshheading:10400065-Apoptosis,
pubmed-meshheading:10400065-Benzodiazepines,
pubmed-meshheading:10400065-Benzothiadiazines,
pubmed-meshheading:10400065-Cells, Cultured,
pubmed-meshheading:10400065-Glutamic Acid,
pubmed-meshheading:10400065-Mice,
pubmed-meshheading:10400065-Neocortex,
pubmed-meshheading:10400065-Neurons,
pubmed-meshheading:10400065-Neuroprotective Agents,
pubmed-meshheading:10400065-Receptors, AMPA
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pubmed:year |
1999
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pubmed:articleTitle |
Cyclothiazide and GYKI 52466 modulate AMPA receptor-mediated apoptosis in cortical neuronal cultures.
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pubmed:affiliation |
Department of Pharmacology, Monash University, Clayton, Victoria, Australia.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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