|
rdf:type |
|
|
lifeskim:mentions |
umls-concept:C0007600,
umls-concept:C0020205,
umls-concept:C0040715,
umls-concept:C0086418,
umls-concept:C0205369,
umls-concept:C0206651,
umls-concept:C0599718,
umls-concept:C0599813,
umls-concept:C0599893,
umls-concept:C0808901,
umls-concept:C1515719,
umls-concept:C1519595,
umls-concept:C1522702,
umls-concept:C1880022
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|
pubmed:issue |
5
|
|
pubmed:dateCreated |
2000-3-30
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|
pubmed:abstractText |
Only a small number of clear cell sarcoma (CCS) cell lines have been reported, including the cell line HS-MM. In the present study, this cell line, maintained for more than 4 years since establishment, was further characterized by cytogenetic studies, fluorescence in situ hybridization (FISH) analysis, and reverse transcriptase-polymerase chain reaction (RT-PCR). HS-MM cells both in vitro and in vivo exhibited pseudodiploid karyotypes with the specific t(12;22)(q13;q12) translocation. The translocation between chromosomes 12 and 22 was confirmed by FISH analysis and the hybrid EWS/ATF-1 transcript induced by this translocation was detected by RT-PCR. The HS-MM cell line will be useful for further studies of CCS.
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pubmed:language |
eng
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pubmed:journal |
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|
pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0022-3417
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 1999 John Wiley & Sons, Ltd.
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pubmed:issnType |
Print
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pubmed:volume |
187
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
594-7
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pubmed:dateRevised |
2004-11-17
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pubmed:meshHeading |
pubmed-meshheading:10398127-Chromosome Aberrations,
pubmed-meshheading:10398127-Chromosomes, Human, Pair 12,
pubmed-meshheading:10398127-Chromosomes, Human, Pair 22,
pubmed-meshheading:10398127-Humans,
pubmed-meshheading:10398127-In Situ Hybridization, Fluorescence,
pubmed-meshheading:10398127-Karyotyping,
pubmed-meshheading:10398127-Oncogene Proteins, Fusion,
pubmed-meshheading:10398127-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:10398127-Sarcoma, Clear Cell,
pubmed-meshheading:10398127-Transcription Factors,
pubmed-meshheading:10398127-Translocation, Genetic,
pubmed-meshheading:10398127-Tumor Cells, Cultured
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|
pubmed:year |
1999
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|
pubmed:articleTitle |
Further characterization of the human clear cell sarcoma cell line HS-MM demonstrating a specific t(12;22)(q13;q12) translocation and hybrid EWS/ATF-1 transcript.
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pubmed:affiliation |
Department of Pathology, Kochi Medical School, Nankoku, Kochi, Japan.
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pubmed:publicationType |
Journal Article
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