Source:http://linkedlifedata.com/resource/pubmed/id/10396290
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1999-7-22
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| pubmed:abstractText |
Chronic myeloproliferative diseases (MPDs) are not associated with consistent cytogenetic or molecular abnormalities. Demonstration of clonal cell growth by analysis of X-chromosome inactivation (XCI) patterns in females provides a promising tool for diagnosis. However, this technique can be complicated by excessive lyonization of normal cells mimicking clonal cell growth: We analyzed XCI patterns at the human androgen receptor (HUMARA) locus in 146 healthy females, 65 women with secondary neutrophilia, 31 women with reactive thrombocytosis, and 86 women with chronic MPDs. A skewed XCI pattern with greater than 75% amplification of 1 allele (allele ratio > 3:1) was found in 22 (9.1%) of 242 control subjects. The incidence of skewing was statistically significantly lower in women younger than 30 years (2/73) compared with women older than 60 years (10/53). Of 86 patients with a chronic MPD, 71 (82%) exhibited an allele ratio greater than 3:1, whereas only 10 (12%) of 86 age-matched control subjects showed a skewed XCI pattern. Although statistical evaluation of the data showed a significant difference between patients with a chronic MPD and control subjects, proof of clonality in individual, especially elderly, patients is difficult.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jul
|
| pubmed:issn |
0002-9173
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
112
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
93-100
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| pubmed:dateRevised |
2008-11-21
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| pubmed:meshHeading |
pubmed-meshheading:10396290-Adolescent,
pubmed-meshheading:10396290-Adult,
pubmed-meshheading:10396290-Aged,
pubmed-meshheading:10396290-Aged, 80 and over,
pubmed-meshheading:10396290-Alleles,
pubmed-meshheading:10396290-Chronic Disease,
pubmed-meshheading:10396290-Clone Cells,
pubmed-meshheading:10396290-Cohort Studies,
pubmed-meshheading:10396290-DNA Primers,
pubmed-meshheading:10396290-Dosage Compensation, Genetic,
pubmed-meshheading:10396290-Female,
pubmed-meshheading:10396290-Humans,
pubmed-meshheading:10396290-Leukemia, Myelogenous, Chronic, BCR-ABL Positive,
pubmed-meshheading:10396290-Leukocytosis,
pubmed-meshheading:10396290-Middle Aged,
pubmed-meshheading:10396290-Myeloproliferative Disorders,
pubmed-meshheading:10396290-Neutrophils,
pubmed-meshheading:10396290-Polycythemia Vera,
pubmed-meshheading:10396290-Polymerase Chain Reaction,
pubmed-meshheading:10396290-Primary Myelofibrosis,
pubmed-meshheading:10396290-Receptors, Androgen,
pubmed-meshheading:10396290-Thrombocytosis
|
| pubmed:year |
1999
|
| pubmed:articleTitle |
Clonality analysis using X-chromosome inactivation at the human androgen receptor gene (Humara). Evaluation of large cohorts of patients with chronic myeloproliferative diseases, secondary neutrophilia, and reactive thrombocytosis.
|
| pubmed:affiliation |
Department of Laboratory Medicine, University of Vienna, Austria.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|