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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1999-7-29
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pubmed:abstractText |
Along humoral immune responses, different stimuli drive the differentiation of B lymphocytes to Ig-secreting plasma cells in discrete microenvironments. The Blimp-1 transcription factor is up-regulated early during the transition of mature B cells to IgM-secreting plasma cells. In the present study, we have examined the requirement of Blimp-1 in plasma cell formation after both T cell-independent (LPS) and -dependent (CD40 + IL-4, Th cell lines) stimulation of spleen B cells. B lymphocyte-induced maturation protein (Blimp-1) was expressed early after in vitro LPS stimulation, mainly in a population of IgM+Syndecan+CD43+ preplasma cells. In contrast, the BSAP transcription factor expressed in mature B cells was down-regulated during the differentiation to plasma cells. Treatment of these cultures with Blimp-1-specific antisense phosphorothioate oligonucleotides suppressed both Blimp-1 protein levels and the emergence of IgM+Syndecan+ cells and plasma cells. However, T-B cell cocultures of spleen B cells from C3H/HeJ (H-2k) mice and syngeneic autoreactive SR.10 Th2 cells submitted to the anti-Blimp-1 therapy did not show any significant reduction in IgM- and IgG1-secreting plasma cell formation. Spleen B cells treated with anti-CD40 mAb + IL-4 differentiated to IgG1-secreting cells without significant transcription of the Blimp-1 gene; anti-Blimp-1 treatment subsequently did not have any effect in the later cultures. Altogether, these results suggest that Blimp-1 transcription factor specifically promotes T cell-independent B cell differentiation to plasma cells, probably at preplasma cell stages. In contrast, T cell-dependent plasma cell formation likely evolves through Blimp-1-independent pathways.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD40,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD43,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin G,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin M,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-4,
http://linkedlifedata.com/resource/pubmed/chemical/Lipopolysaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Prdm1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Proteoglycans,
http://linkedlifedata.com/resource/pubmed/chemical/Repressor Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Sialoglycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Spn protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Syndecans,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0022-1767
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
163
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
611-7
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:10395648-Animals,
pubmed-meshheading:10395648-Antibodies, Monoclonal,
pubmed-meshheading:10395648-Antibody-Producing Cells,
pubmed-meshheading:10395648-Antigens, CD,
pubmed-meshheading:10395648-Antigens, CD40,
pubmed-meshheading:10395648-Antigens, CD43,
pubmed-meshheading:10395648-B-Lymphocytes,
pubmed-meshheading:10395648-Cell Differentiation,
pubmed-meshheading:10395648-Cell Line,
pubmed-meshheading:10395648-Immunoglobulin Class Switching,
pubmed-meshheading:10395648-Immunoglobulin G,
pubmed-meshheading:10395648-Immunoglobulin M,
pubmed-meshheading:10395648-Interleukin-4,
pubmed-meshheading:10395648-Lipopolysaccharides,
pubmed-meshheading:10395648-Lymphocyte Activation,
pubmed-meshheading:10395648-Membrane Glycoproteins,
pubmed-meshheading:10395648-Mice,
pubmed-meshheading:10395648-Mice, Inbred BALB C,
pubmed-meshheading:10395648-Mice, Inbred C3H,
pubmed-meshheading:10395648-Plasma Cells,
pubmed-meshheading:10395648-Proteoglycans,
pubmed-meshheading:10395648-Repressor Proteins,
pubmed-meshheading:10395648-Sialoglycoproteins,
pubmed-meshheading:10395648-Stem Cells,
pubmed-meshheading:10395648-Syndecans,
pubmed-meshheading:10395648-T-Lymphocytes,
pubmed-meshheading:10395648-T-Lymphocytes, Helper-Inducer,
pubmed-meshheading:10395648-Transcription Factors,
pubmed-meshheading:10395648-Up-Regulation
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pubmed:year |
1999
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pubmed:articleTitle |
Differential involvement of the transcription factor Blimp-1 in T cell-independent and -dependent B cell differentiation to plasma cells.
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pubmed:affiliation |
Centro Nacional de Biología Fundamental, Instituto de Salud Carlos III, Majadahonda, Spain.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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