| pubmed-article:10395294 | rdf:type | pubmed:Citation | lld:pubmed |
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| pubmed-article:10395294 | lifeskim:mentions | umls-concept:C1332737 | lld:lifeskim |
| pubmed-article:10395294 | lifeskim:mentions | umls-concept:C1705523 | lld:lifeskim |
| pubmed-article:10395294 | lifeskim:mentions | umls-concept:C0392756 | lld:lifeskim |
| pubmed-article:10395294 | lifeskim:mentions | umls-concept:C0021469 | lld:lifeskim |
| pubmed-article:10395294 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:10395294 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:10395294 | lifeskim:mentions | umls-concept:C0333668 | lld:lifeskim |
| pubmed-article:10395294 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:10395294 | pubmed:dateCreated | 1999-7-16 | lld:pubmed |
| pubmed-article:10395294 | pubmed:abstractText | In this study, we have evaluated the effects of extracellular magnesium restriction on the growth and cell cycle parameters of normal (HC11) and transformed (MCF-7) breast epithelial cell lines. Cells were incubated in medium with different concentrations of Mg2+ (from 0.5 to 0 mM) and the growth rates were determined by [3H]-thymidine incorporation and cell counting. The growth of the HC11 cells was drastically inhibited by Mg2+ depletion whereas the MCF-7 cells were only slightly inhibited (about 50% and 15%, respectively, after incubation in 0.05 mM Mg for 48 h). Cell cycle analyses showed a decrease in the percentage of cells in the S phase when both cell lines were incubated at low Mg2+ concentration. However, while the percentage of cells in both the G0/G1 and G2/M phases was increased in the HC11 cells, only the percentage of cells in the G2/M phase was increased in the MCF-7 cell line. Extracellular magnesium depletion was associated with increased expression of the cyclin-dependent kinase inhibitor p27Kip1 and decreased expression of cyclin D1 in the HC11 but not in the MCF-7 cells. We also demonstrated that Mg2+ depletion does not inhibit kinase activities in the normal HC11 cells and that Mg2+-restricted HC11 cells are still responsive to the epidermal growth factor (EGF)- and insulin-mediated stimulation of cell growth. These data suggest that normal but not transformed mammary epithelial cells are inhibited by extracellular Mg2+ restriction and that this effect might be mediated by changes in the levels of expression of both cyclin D1 and p27Kip1. | lld:pubmed |
| pubmed-article:10395294 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10395294 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10395294 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:10395294 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:10395294 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:10395294 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10395294 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10395294 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:10395294 | pubmed:issn | 0021-9541 | lld:pubmed |
| pubmed-article:10395294 | pubmed:author | pubmed-author:CittadiniAA | lld:pubmed |
| pubmed-article:10395294 | pubmed:author | pubmed-author:WolfF IFI | lld:pubmed |
| pubmed-article:10395294 | pubmed:author | pubmed-author:SgambatoAA | lld:pubmed |
| pubmed-article:10395294 | pubmed:author | pubmed-author:FaragliaBB | lld:pubmed |
| pubmed-article:10395294 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10395294 | pubmed:volume | 180 | lld:pubmed |
| pubmed-article:10395294 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10395294 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10395294 | pubmed:pagination | 245-54 | lld:pubmed |
| pubmed-article:10395294 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
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| pubmed-article:10395294 | pubmed:meshHeading | pubmed-meshheading:10395294... | lld:pubmed |
| pubmed-article:10395294 | pubmed:year | 1999 | lld:pubmed |
| pubmed-article:10395294 | pubmed:articleTitle | Magnesium depletion causes growth inhibition, reduced expression of cyclin D1, and increased expression of P27Kip1 in normal but not in transformed mammary epithelial cells. | lld:pubmed |
| pubmed-article:10395294 | pubmed:affiliation | Centro di Ricerche Oncologiche Giovanni XXIII, Istituto di Patologia Generale, Università Cattolica del Sacro Cuore, Rome, Italy. ibipg@rm.unicatt.it | lld:pubmed |
| pubmed-article:10395294 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:10395294 | pubmed:publicationType | Comparative Study | lld:pubmed |
| pubmed-article:10395294 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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