Linked Life Data

10395294

Source:http://linkedlifedata.com/resource/pubmed/id/10395294

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1999-7-16
pubmed:abstractText
In this study, we have evaluated the effects of extracellular magnesium restriction on the growth and cell cycle parameters of normal (HC11) and transformed (MCF-7) breast epithelial cell lines. Cells were incubated in medium with different concentrations of Mg2+ (from 0.5 to 0 mM) and the growth rates were determined by [3H]-thymidine incorporation and cell counting. The growth of the HC11 cells was drastically inhibited by Mg2+ depletion whereas the MCF-7 cells were only slightly inhibited (about 50% and 15%, respectively, after incubation in 0.05 mM Mg for 48 h). Cell cycle analyses showed a decrease in the percentage of cells in the S phase when both cell lines were incubated at low Mg2+ concentration. However, while the percentage of cells in both the G0/G1 and G2/M phases was increased in the HC11 cells, only the percentage of cells in the G2/M phase was increased in the MCF-7 cell line. Extracellular magnesium depletion was associated with increased expression of the cyclin-dependent kinase inhibitor p27Kip1 and decreased expression of cyclin D1 in the HC11 but not in the MCF-7 cells. We also demonstrated that Mg2+ depletion does not inhibit kinase activities in the normal HC11 cells and that Mg2+-restricted HC11 cells are still responsive to the epidermal growth factor (EGF)- and insulin-mediated stimulation of cell growth. These data suggest that normal but not transformed mammary epithelial cells are inhibited by extracellular Mg2+ restriction and that this effect might be mediated by changes in the levels of expression of both cyclin D1 and p27Kip1.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0021-9541
pubmed:author
pubmed:issnType
Print
pubmed:volume
180
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
245-54
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:10395294-Animals, pubmed-meshheading:10395294-Breast Neoplasms, pubmed-meshheading:10395294-Cell Cycle Proteins, pubmed-meshheading:10395294-Cell Division, pubmed-meshheading:10395294-Cell Line, Transformed, pubmed-meshheading:10395294-Cyclin D1, pubmed-meshheading:10395294-Cyclin E, pubmed-meshheading:10395294-Cyclin-Dependent Kinase Inhibitor p27, pubmed-meshheading:10395294-Enzyme Inhibitors, pubmed-meshheading:10395294-Female, pubmed-meshheading:10395294-Flow Cytometry, pubmed-meshheading:10395294-G0 Phase, pubmed-meshheading:10395294-Gene Expression Regulation, Neoplastic, pubmed-meshheading:10395294-Humans, pubmed-meshheading:10395294-Magnesium, pubmed-meshheading:10395294-Mammary Glands, Animal, pubmed-meshheading:10395294-Mice, pubmed-meshheading:10395294-Mice, Inbred BALB C, pubmed-meshheading:10395294-Microtubule-Associated Proteins, pubmed-meshheading:10395294-Pregnancy, pubmed-meshheading:10395294-S Phase, pubmed-meshheading:10395294-Thymidine, pubmed-meshheading:10395294-Tritium, pubmed-meshheading:10395294-Tumor Suppressor Proteins
pubmed:year
1999
pubmed:articleTitle
Magnesium depletion causes growth inhibition, reduced expression of cyclin D1, and increased expression of P27Kip1 in normal but not in transformed mammary epithelial cells.
pubmed:affiliation
Centro di Ricerche Oncologiche Giovanni XXIII, Istituto di Patologia Generale, Università Cattolica del Sacro Cuore, Rome, Italy. ibipg@rm.unicatt.it
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't