10394917

Source:http://linkedlifedata.com/resource/pubmed/id/10394917

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Predicate	Object
rdf:type	pubmed:Citation
lifeskim:mentions	umls-concept:C0025663, umls-concept:C0037119, umls-concept:C0185125, umls-concept:C0205352, umls-concept:C0443131, umls-concept:C0596607, umls-concept:C0796345, umls-concept:C2745888
pubmed:issue	4-5
pubmed:dateCreated	1999-7-30
pubmed:abstractText	A simple and sensitive method for linkage analysis is described, which is based on conformation-sensitive gel electrophoresis (CSGE). Using urea-containing agarose gels or a commercially available polyacrylamide-derived matrix, 13 polymorphic markers were newly identified for known genes of the silkworm, Bombyx mori, which had been scored as monomorphic by PCR-RFLP analysis. This method for detecting polymorphisms is quite sensitive, and can be performed with inexpensive reagents and apparatus that is available in most molecular biology laboratories.
pubmed:language	eng
pubmed:journal	http://linkedlifedata.com/resource/pubmed/journal/0125036
pubmed:citationSubset	IM
pubmed:chemical	http://linkedlifedata.com/resource/pubmed/chemical/DNA, http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers, http://linkedlifedata.com/resource/pubmed/chemical/Genetic Markers, http://linkedlifedata.com/resource/pubmed/chemical/Insect Proteins
pubmed:status	MEDLINE
pubmed:month	Jun
pubmed:issn	0026-8925
pubmed:author	pubmed-author:YasukochiYY
pubmed:issnType	Print
pubmed:volume	261
pubmed:owner	NLM
pubmed:authorsComplete	Y
pubmed:pagination	796-802
pubmed:dateRevised	2010-11-18
pubmed:meshHeading	pubmed-meshheading:10394917-Animals, pubmed-meshheading:10394917-Base Sequence, pubmed-meshheading:10394917-Bombyx, pubmed-meshheading:10394917-Chromosome Mapping, pubmed-meshheading:10394917-Cloning, Molecular, pubmed-meshheading:10394917-Crosses, Genetic, pubmed-meshheading:10394917-DNA, pubmed-meshheading:10394917-DNA Primers, pubmed-meshheading:10394917-Databases as Topic, pubmed-meshheading:10394917-Electrophoresis, pubmed-meshheading:10394917-Female, pubmed-meshheading:10394917-Genes, Dominant, pubmed-meshheading:10394917-Genetic Linkage, pubmed-meshheading:10394917-Genetic Markers, pubmed-meshheading:10394917-Insect Proteins, pubmed-meshheading:10394917-Male, pubmed-meshheading:10394917-Nucleic Acid Conformation, pubmed-meshheading:10394917-Polymerase Chain Reaction, pubmed-meshheading:10394917-Polymorphism, Restriction Fragment Length, pubmed-meshheading:10394917-Sensitivity and Specificity, pubmed-meshheading:10394917-Sequence Tagged Sites
pubmed:year	1999
pubmed:articleTitle	A simple and accurate method for generating co-dominant markers: an application of conformation-sensitive gel electrophoresis to linkage analysis in the silkworm.
pubmed:affiliation	National Institute of Sericultural and Entomological Science, Tsukuba, Ibaraki, Japan. yaskoch@nises.affrc.go.jp
pubmed:publicationType	Journal Article, Research Support, Non-U.S. Gov't