|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
1
|
|
pubmed:dateCreated |
1999-7-26
|
|
pubmed:abstractText |
Based on the finding that gene expression for the actin-bundling protein L-plastin is inducible by androgen and that L-plastin is overexpressed in malignant epithelium of the prostate, we examined the functional consequences of L-plastin down-regulation in prostate carcinoma cell lines by both transfection and retroviral infection. We constructed retroviral vectors to express two different regions of the L-plastin gene, a 1713-bp 3'-coding portion and a 163-bp 5'-untranslated region, both in antisense orientation. Introduction of either constructs into prostate carcinoma cell lines, PC-3 and its isogenic but metastatic variant PC-3M cells, reduced the growth rates of both cell lines. In vitro invasion and motility of PC-3 and PC-3M cells were drastically suppressed (approximately 10-fold) by the expression of the antisense constructs. Evidence was obtained to indicate that L-plastin protein levels were indeed decreased by the antisense expression. The antisense construct for the 5'-untranslated region with the most unique sequence for the L-plastin gene was more effective in down-regulation efficiency compared with the larger antisense construct in the coding region, which maintains homology to other members of the plastin gene family. Cells infected with the 163-bp antisense virus, which were also tested in a nude mouse diaphragm invasion model, showed suppression of in vivo invasion of both PC-3 and PC-3M cells. These results suggested that overexpression of L-plastin might be functionally involved in prostate cancer invasion and metastasis, and raised the possibility that L-plastin gene-specific antisense delivery could potentially be a useful approach to interfere with prostate cancer progression in vivo.
|
|
pubmed:grant |
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/10393844-1419062,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10393844-1491005,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10393844-1904011,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10393844-2058002,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10393844-2391360,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10393844-4053036,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10393844-7558435,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10393844-7806577,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10393844-7958846,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10393844-8137292,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10393844-8139549,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10393844-8417833,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10393844-8428952,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10393844-8461306,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10393844-8689569,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10393844-8940338,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10393844-8946830,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10393844-9176394,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10393844-9525747,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10393844-9689080
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
AIM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jul
|
|
pubmed:issn |
0002-9440
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
155
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
115-22
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:10393844-Animals,
pubmed-meshheading:10393844-Antisense Elements (Genetics),
pubmed-meshheading:10393844-Carcinoma,
pubmed-meshheading:10393844-Cell Division,
pubmed-meshheading:10393844-Cell Movement,
pubmed-meshheading:10393844-Male,
pubmed-meshheading:10393844-Membrane Glycoproteins,
pubmed-meshheading:10393844-Mice,
pubmed-meshheading:10393844-Mice, Nude,
pubmed-meshheading:10393844-Microfilament Proteins,
pubmed-meshheading:10393844-Neoplasm Invasiveness,
pubmed-meshheading:10393844-Phosphoproteins,
pubmed-meshheading:10393844-Prostatic Neoplasms
|
|
pubmed:year |
1999
|
|
pubmed:articleTitle |
Suppression of prostate carcinoma cell invasion by expression of antisense L-plastin gene.
|
|
pubmed:affiliation |
Departments of Pathology, University of Southern California School of Medicine, Los Angeles, California, USA.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|
